Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   F9800_RS27935 Genome accession   NZ_CP045030
Coordinates   4247443..4248486 (-) Length   347 a.a.
NCBI ID   WP_001013181.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain JW-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4242443..4253486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9800_RS27890 (F9800_27920) - 4242876..4243076 (-) 201 WP_000106083.1 YqzE family protein -
  F9800_RS27895 (F9800_27925) aroK 4243115..4243612 (-) 498 WP_000836635.1 shikimate kinase AroK -
  F9800_RS27900 (F9800_27930) - 4243728..4244378 (-) 651 WP_000183866.1 2OG-Fe(II) oxygenase -
  F9800_RS27905 (F9800_27935) comGG 4244553..4244924 (-) 372 WP_001214886.1 competence type IV pilus minor pilin ComGG -
  F9800_RS27910 (F9800_27940) comGF 4244921..4245391 (-) 471 WP_000923110.1 competence type IV pilus minor pilin ComGF -
  F9800_RS27915 (F9800_27945) - 4245361..4245663 (-) 303 WP_000829465.1 hypothetical protein -
  F9800_RS27920 (F9800_27950) comGD 4245656..4246111 (-) 456 WP_003257968.1 competence type IV pilus minor pilin ComGD -
  F9800_RS27925 (F9800_27955) comGC 4246108..4246407 (-) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  F9800_RS27930 (F9800_27960) comGB 4246419..4247456 (-) 1038 WP_003257965.1 competence type IV pilus assembly protein ComGB -
  F9800_RS27935 (F9800_27965) comGA 4247443..4248486 (-) 1044 WP_001013181.1 competence type IV pilus ATPase ComGA Machinery gene
  F9800_RS27940 (F9800_27970) - 4248692..4249387 (+) 696 WP_000434108.1 helix-turn-helix transcriptional regulator -
  F9800_RS27945 (F9800_27975) - 4249514..4249756 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  F9800_RS27950 (F9800_27980) - 4249864..4251258 (+) 1395 WP_001094344.1 L-cystine transporter -
  F9800_RS27955 (F9800_27985) - 4251338..4251730 (-) 393 WP_000877906.1 hypothetical protein -
  F9800_RS27960 (F9800_27990) - 4251818..4252033 (-) 216 WP_001008326.1 DUF3912 family protein -
  F9800_RS27965 (F9800_27995) - 4252287..4252598 (+) 312 WP_001093240.1 hypothetical protein -
  F9800_RS27970 (F9800_28000) - 4252633..4253121 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39168.55 Da        Isoelectric Point: 8.7212

>NTDB_id=391333 F9800_RS27935 WP_001013181.1 4247443..4248486(-) (comGA) [Bacillus thuringiensis strain JW-1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=391333 F9800_RS27935 WP_001013181.1 4247443..4248486(-) (comGA) [Bacillus thuringiensis strain JW-1]
ATGAATGGGATTGAAATTTTTGCTAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment