Detailed information NTG0391294

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F9865_RS21300
• Genome accession: NZ_CP045015
• Coordinates: 4269311..4270111 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain BK13048
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4264311..4275111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F9865_RS21265 (F9865_21275)	gspH	4264467..4264982 (+)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-
F9865_RS21270 (F9865_21280)	gspI	4264979..4265344 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
F9865_RS21275 (F9865_21285)	gspJ	4265341..4265937 (+)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
F9865_RS21280 (F9865_21290)	gspK	4265934..4266920 (+)	987	WP_077254844.1	type II secretion system minor pseudopilin GspK	-
F9865_RS21285 (F9865_21295)	gspL	4266907..4268100 (+)	1194	WP_032428212.1	type II secretion system protein GspL	-
F9865_RS21290 (F9865_21300)	-	4268093..4268578 (+)	486	WP_004151943.1	type II secretion system protein M	-
F9865_RS21295 (F9865_21305)	-	4268568..4269314 (+)	747	WP_023316460.1	type II secretion system protein N	-
F9865_RS21300 (F9865_21310)	pilD	4269311..4270111 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
F9865_RS21305 (F9865_21315)	hrpB	4270159..4272588 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
F9865_RS21310 (F9865_21320)	thpR	4272661..4273197 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
F9865_RS21315 (F9865_21325)	sfsA	4273197..4273913 (+)	717	WP_023342992.1	DNA/RNA nuclease SfsA	-
F9865_RS21320 (F9865_21330)	dksA	4274076..4274531 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=391294 F9865_RS21300 WP_004145898.1 4269311..4270111(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain BK13048]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=391294 F9865_RS21300 WP_004145898.1 4269311..4270111(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain BK13048]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425