Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DDE73_RS25340 Genome accession   NZ_CP044978
Coordinates   4899751..4900794 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus thuringiensis strain BT62     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4894751..4905794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDE73_RS25295 (DDE73_25305) - 4895184..4895384 (-) 201 WP_000106083.1 YqzE family protein -
  DDE73_RS25300 (DDE73_25310) - 4895423..4895920 (-) 498 WP_103622332.1 shikimate kinase -
  DDE73_RS25305 (DDE73_25315) - 4896036..4896686 (-) 651 WP_048655624.1 2OG-Fe(II) oxygenase -
  DDE73_RS25310 (DDE73_25320) comGG 4896861..4897232 (-) 372 WP_025687689.1 competence type IV pilus minor pilin ComGG -
  DDE73_RS25315 (DDE73_25325) comGF 4897229..4897699 (-) 471 WP_142391051.1 competence type IV pilus minor pilin ComGF -
  DDE73_RS25320 (DDE73_25330) - 4897669..4897971 (-) 303 WP_103622330.1 competence protein ComG -
  DDE73_RS25325 (DDE73_25335) comGD 4897964..4898419 (-) 456 WP_103622329.1 competence type IV pilus minor pilin ComGD -
  DDE73_RS25330 (DDE73_25340) comGC 4898416..4898715 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  DDE73_RS25335 (DDE73_25345) comGB 4898727..4899758 (-) 1032 WP_080470803.1 competence type IV pilus assembly protein ComGB -
  DDE73_RS25340 (DDE73_25350) comGA 4899751..4900794 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  DDE73_RS25345 (DDE73_25355) - 4901000..4901695 (+) 696 WP_103622328.1 helix-turn-helix domain-containing protein -
  DDE73_RS25350 (DDE73_25360) - 4901821..4902063 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  DDE73_RS25355 (DDE73_25365) - 4902171..4903565 (+) 1395 WP_103622327.1 L-cystine transporter -
  DDE73_RS25360 (DDE73_25370) - 4903644..4904036 (-) 393 WP_000875394.1 hypothetical protein -
  DDE73_RS25365 (DDE73_25375) - 4904124..4904339 (-) 216 WP_001008327.1 DUF3912 family protein -
  DDE73_RS25370 (DDE73_25380) - 4904592..4904903 (+) 312 WP_001093237.1 hypothetical protein -
  DDE73_RS25375 (DDE73_25385) - 4904938..4905426 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=391122 DDE73_RS25340 WP_001013170.1 4899751..4900794(-) (comGA) [Bacillus thuringiensis strain BT62]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=391122 DDE73_RS25340 WP_001013170.1 4899751..4900794(-) (comGA) [Bacillus thuringiensis strain BT62]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372

Multiple sequence alignment