Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   F8M43_RS12665 Genome accession   NZ_CP044498
Coordinates   2442076..2443146 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain ms-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437076..2448146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS12620 tapA 2437555..2438316 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  F8M43_RS12625 yqzG 2438587..2438913 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  F8M43_RS12630 spoIITA 2438955..2439134 (-) 180 WP_029726723.1 YqzE family protein -
  F8M43_RS12635 comGG 2439206..2439580 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  F8M43_RS12640 comGF 2439581..2439964 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  F8M43_RS12645 comGE 2439990..2440337 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  F8M43_RS12650 comGD 2440321..2440752 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  F8M43_RS12655 comGC 2440742..2441038 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  F8M43_RS12660 comGB 2441052..2442089 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  F8M43_RS12665 comGA 2442076..2443146 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  F8M43_RS12670 - 2443359..2443556 (-) 198 WP_029726717.1 hypothetical protein -
  F8M43_RS12675 corA 2443558..2444511 (-) 954 WP_151433387.1 magnesium transporter CorA -
  F8M43_RS12680 yqhB 2444654..2445982 (+) 1329 WP_029726716.1 hemolysin family protein -
  F8M43_RS12685 rsbRD 2446035..2446871 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  F8M43_RS12695 mgsR 2447095..2447475 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  F8M43_RS12700 yqgY 2447707..2447952 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=390570 F8M43_RS12665 WP_004399124.1 2442076..2443146(-) (comGA) [Bacillus subtilis strain ms-2]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=390570 F8M43_RS12665 WP_004399124.1 2442076..2443146(-) (comGA) [Bacillus subtilis strain ms-2]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment