Detailed information NTG0388980

Bioinformatically predicted

Overview

• Name: comFA/cflA
• Type: Machinery gene
• Locus tag: LPB220_RS02850
• Genome accession: NZ_CP044230
• Coordinates: 537403..538701 (+)
• Length: 432 a.a.
• NCBI ID: WP_061591378.1
• Uniprot ID: A0A5J6LKN8
• Organism: Streptococcus sp. LPB0220
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	535287..536635	537403..538701	flank	768

Gene organization within MGE regions

Location: 535287..538701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPB220_RS02845 (LPB220_02845)	-	536706..537347 (-)	642	WP_070664862.1	YigZ family protein	-
LPB220_RS02850 (LPB220_02850)	comFA/cflA	537403..538701 (+)	1299	WP_061591378.1	DEAD/DEAH box helicase	Machinery gene

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 49393.47 Da
• Isoelectric Point: 9.3591

>NTDB_id=388980 LPB220_RS02850 WP_061591378.1 537403..538701(+) (comFA/cflA) [Streptococcus sp. LPB0220]
MKIEDSYGRLLTESQMTNDLKEIAQELPAIEKQNGRYQCFRCGSMIDQKLWKLSEEVLYCRACIQLGRIRSDQKLYAIAQ
QDFEGQEVLNWKGTLTSYQQEVSEGLIQAVKAGKHALVHAVTGAGKTEMMYQVVATAIKAGKAVCIATPRIDVCIELYGR
MKDDFSCPISLLHGESEPYFRTPLVIATTHQLLKFYQAFDLLIIDEVDAFPYVDNPILYKAAQNAIKKEGNTLYLTATST
DELDKKVKKKEIIRYSLPRRFHGNPLVVPEIKWVPKIREKIEKGRIPYELLQLIKKQRQTHYPLLIFVSEIELGQQFTEN
LKKYFPKETVGFVSSQTTDRLRMVEKFRNKAITMLVSTTILERGVTFPFVDVFVLESNHKLFTKSALVQISGRVGRSKER
PTGELLFLSDGITRGMKKAIKEIKEMNQEAGF

Nucleotide

• Length: 1299 bp

>NTDB_id=388980 LPB220_RS02850 WP_061591378.1 537403..538701(+) (comFA/cflA) [Streptococcus sp. LPB0220]
ATGAAGATAGAAGATTCTTATGGAAGACTCTTAACGGAGAGTCAAATGACAAATGATCTAAAAGAAATTGCTCAAGAACT
TCCAGCTATAGAAAAACAGAATGGAAGGTACCAATGCTTTCGATGTGGCAGTATGATTGATCAAAAACTGTGGAAGTTGA
GTGAAGAGGTACTGTATTGTAGAGCCTGTATCCAATTAGGAAGGATCCGGAGTGATCAAAAACTTTATGCTATTGCACAG
CAAGACTTTGAAGGTCAAGAGGTGCTAAACTGGAAAGGGACCTTGACTTCCTATCAACAAGAGGTATCTGAAGGACTTAT
CCAAGCAGTTAAAGCAGGAAAACATGCCTTGGTACATGCTGTGACAGGAGCTGGAAAAACGGAAATGATGTATCAGGTTG
TAGCAACAGCTATCAAGGCAGGAAAAGCAGTCTGTATTGCTACCCCAAGAATCGATGTCTGTATCGAACTGTACGGAAGA
ATGAAGGATGATTTTTCCTGCCCCATCTCTTTGCTTCATGGAGAATCGGAGCCCTATTTTAGAACGCCTTTAGTGATCGC
TACAACCCACCAATTGCTAAAGTTTTATCAGGCTTTTGATCTCCTTATTATAGATGAGGTAGATGCTTTTCCCTATGTGG
ATAATCCAATCTTATATAAAGCAGCTCAGAATGCAATAAAAAAAGAGGGGAATACCCTATATTTGACAGCAACCTCTACA
GATGAGCTAGATAAAAAGGTTAAGAAAAAAGAAATCATTCGTTATAGTCTTCCAAGAAGATTTCATGGGAACCCACTAGT
GGTCCCTGAAATAAAATGGGTGCCGAAAATTAGAGAAAAAATTGAAAAAGGAAGAATCCCTTACGAACTATTGCAACTGA
TCAAGAAACAAAGACAGACTCACTACCCATTGTTAATCTTTGTATCTGAAATAGAATTAGGACAACAATTCACTGAGAAC
TTAAAAAAATACTTTCCCAAAGAAACAGTAGGTTTTGTATCCTCACAGACAACAGACCGCCTACGAATGGTAGAAAAGTT
TAGAAACAAAGCCATAACCATGCTAGTCTCGACAACAATTTTAGAAAGAGGAGTGACTTTTCCATTTGTAGATGTCTTTG
TTTTAGAAAGTAATCACAAATTATTTACTAAAAGTGCCCTCGTACAAATTTCAGGAAGGGTCGGTCGAAGTAAAGAAAGA
CCAACAGGTGAACTACTTTTTCTATCAGATGGTATAACACGAGGAATGAAAAAAGCGATTAAAGAAATAAAGGAAATGAA
TCAGGAGGCTGGATTTTGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (94-238)

• Helicase conserved C-terminal domain (Helicase_C) (290-397)

3D structure

• AlphaFold DB: A0A5J6LKN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA/cflA	Streptococcus pneumoniae Rx1	60.508	100	0.606
comFA/cflA	Streptococcus pneumoniae D39	60.508	100	0.606
comFA/cflA	Streptococcus pneumoniae R6	60.508	100	0.606
comFA/cflA	Streptococcus mitis SK321	60.508	100	0.606
comFA/cflA	Streptococcus pneumoniae TIGR4	60.277	100	0.604
comFA/cflA	Streptococcus mitis NCTC 12261	59.815	100	0.6
comFA	Lactococcus lactis subsp. cremoris KW2	50.503	92.13	0.465
comFA	Latilactobacillus sakei subsp. sakei 23K	37.413	100	0.375