Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HKJ31_RS04615 Genome accession   NZ_CP052855
Coordinates   1014106..1015806 (+) Length   566 a.a.
NCBI ID   WP_004083688.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain Fillmore     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009106..1020806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKJ31_RS04600 (HKJ31_04685) - 1009341..1009793 (+) 453 WP_004083690.1 TauD/TfdA family dioxygenase -
  HKJ31_RS11495 - 1009790..1010308 (+) 519 WP_004084214.1 hypothetical protein -
  HKJ31_RS11500 - 1010342..1010701 (+) 360 WP_004084115.1 AEC family transporter -
  HKJ31_RS04610 (HKJ31_04695) - 1011366..1013813 (+) 2448 WP_004083689.1 YadA family autotransporter adhesin -
  HKJ31_RS04615 (HKJ31_04700) pilF 1014106..1015806 (+) 1701 WP_004083688.1 type II secretion system ATPase GspE Machinery gene
  HKJ31_RS04620 (HKJ31_04705) - 1015842..1017059 (+) 1218 WP_004083687.1 type II secretion system F family protein -
  HKJ31_RS04625 (HKJ31_04710) gspG 1017412..1017846 (+) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  HKJ31_RS04630 (HKJ31_04715) - 1017852..1018358 (+) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  HKJ31_RS04635 (HKJ31_04720) - 1018355..1018753 (+) 399 WP_004083684.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HKJ31_RS04640 (HKJ31_04725) - 1018750..1019376 (+) 627 WP_004083683.1 type II secretion system protein J -
  HKJ31_RS04645 (HKJ31_04730) - 1019376..1020221 (+) 846 WP_004083682.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62681.47 Da        Isoelectric Point: 5.0845

>NTDB_id=388922 HKJ31_RS04615 WP_004083688.1 1014106..1015806(+) (pilF) [Xylella fastidiosa subsp. multiplex strain Fillmore]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPTHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDVLRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=388922 HKJ31_RS04615 WP_004083688.1 1014106..1015806(+) (pilF) [Xylella fastidiosa subsp. multiplex strain Fillmore]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGTATCAATCAGATCCAGGCTAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACGGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCACAGCGTTTGGTGC
GTCGCTTGGAGCCTACGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATTGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGCTTGCGCCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAGTTTCTGATTATGGATGATGTGCTGCGTCGCGCGGTGATCAGCCGTACCGGCATAGGGGAGTTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACTACAATTGAAGAGGTCTTG
CGCGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.463

99.117

0.401
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

40.074

95.23

0.382
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369