Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   F5K02_RS01815 Genome accession   NZ_CP044132
Coordinates   371514..371951 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain ZKY01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366514..376951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F5K02_RS01790 (F5K02_01805) - 367534..368670 (+) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -
  F5K02_RS01795 (F5K02_01810) - 368685..369119 (+) 435 WP_007409358.1 RDD family protein -
  F5K02_RS01800 (F5K02_01815) - 369191..369514 (+) 324 WP_007409357.1 YckD family protein -
  F5K02_RS01805 (F5K02_01820) - 369618..371054 (+) 1437 WP_241457875.1 family 1 glycosylhydrolase -
  F5K02_RS01810 (F5K02_01825) nin/comJ 371095..371493 (-) 399 WP_039252825.1 competence protein ComJ Regulator
  F5K02_RS01815 (F5K02_01830) nucA/comI 371514..371951 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  F5K02_RS01820 (F5K02_01835) hxlB 372309..372866 (-) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  F5K02_RS01825 (F5K02_01840) hxlA 372863..373498 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  F5K02_RS01830 (F5K02_01845) - 373730..374092 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=388068 F5K02_RS01815 WP_003156588.1 371514..371951(-) (nucA/comI) [Bacillus amyloliquefaciens strain ZKY01]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=388068 F5K02_RS01815 WP_003156588.1 371514..371951(-) (nucA/comI) [Bacillus amyloliquefaciens strain ZKY01]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment