Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FOB72_RS17110 Genome accession   NZ_CP044065
Coordinates   3715162..3716025 (-) Length   287 a.a.
NCBI ID   WP_150373673.1    Uniprot ID   A0A5P2H7A4
Organism   Cupriavidus pauculus strain FDAARGOS_664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3710162..3721025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB72_RS17085 (FOB72_17080) - 3710800..3711234 (+) 435 WP_150373668.1 NUDIX domain-containing protein -
  FOB72_RS17090 (FOB72_17085) - 3711281..3713332 (+) 2052 WP_150373669.1 OPT family oligopeptide transporter -
  FOB72_RS17095 (FOB72_17090) - 3713345..3713527 (-) 183 WP_150373670.1 DNA gyrase inhibitor YacG -
  FOB72_RS17100 (FOB72_17095) zapD 3713550..3714308 (-) 759 WP_150373671.1 cell division protein ZapD -
  FOB72_RS17105 (FOB72_17100) coaE 3714513..3715115 (-) 603 WP_150373672.1 dephospho-CoA kinase -
  FOB72_RS17110 (FOB72_17105) pilD 3715162..3716025 (-) 864 WP_150373673.1 prepilin peptidase Machinery gene
  FOB72_RS17115 (FOB72_17110) - 3716029..3717300 (-) 1272 WP_150373674.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31056.10 Da        Isoelectric Point: 7.7637

>NTDB_id=387359 FOB72_RS17110 WP_150373673.1 3715162..3716025(-) (pilD) [Cupriavidus pauculus strain FDAARGOS_664]
MLQVSELAALPPAFLVFAAGLIGLVVGSFLNVVIHRIPRMMEYDEANYIAELRGEPLPHPGRYNLVVPRSACPHCGHQIA
AWENIPVVSYLFLRGRCAGCKTRISVRYPLVELASAALSALAMWHYGPTVQALAAIVMVWALIALTMIDADTQLLPDQIT
LPLLWLGLLLNLQGLFVPLADAVIGAAAGYLVLWAAYWLFKLVRGKEGMGYGDFKLMGALGAWFGWQALPALVLLSSVVG
AVFGILMLVVRRQGRETPFPFGPFIAGAGLIVLFFGADLLPLALSIH

Nucleotide


Download         Length: 864 bp        

>NTDB_id=387359 FOB72_RS17110 WP_150373673.1 3715162..3716025(-) (pilD) [Cupriavidus pauculus strain FDAARGOS_664]
ATGCTGCAGGTATCGGAGCTGGCGGCGCTGCCGCCGGCCTTTCTCGTCTTCGCGGCCGGACTGATCGGCCTGGTGGTCGG
CAGCTTCCTCAACGTGGTGATCCACCGGATTCCGCGCATGATGGAATACGACGAGGCGAACTACATCGCCGAGTTGCGGG
GCGAACCTCTGCCCCACCCCGGCCGCTACAACCTCGTGGTGCCGCGCTCGGCGTGTCCGCACTGCGGGCACCAGATCGCG
GCATGGGAGAACATCCCCGTCGTCAGCTATCTGTTCCTGCGCGGCCGCTGCGCGGGCTGCAAGACGCGCATCAGCGTGCG
CTACCCGCTCGTGGAACTGGCCAGCGCCGCGCTCAGCGCGCTGGCGATGTGGCATTACGGCCCGACCGTGCAGGCGCTGG
CCGCCATCGTCATGGTCTGGGCCCTGATCGCGCTCACGATGATCGACGCCGATACCCAGCTGCTGCCGGACCAGATCACG
CTGCCGCTGCTGTGGCTCGGCCTGCTGCTGAACCTCCAGGGCCTGTTCGTGCCGCTCGCCGACGCCGTGATCGGCGCCGC
GGCGGGCTACCTCGTGCTGTGGGCCGCCTACTGGCTGTTCAAGCTCGTGCGGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGCTGATGGGCGCGCTCGGCGCGTGGTTCGGCTGGCAGGCCCTGCCCGCGCTCGTGCTGCTGTCCTCGGTGGTCGGC
GCGGTGTTCGGCATCCTGATGCTCGTCGTGCGCCGCCAGGGACGCGAGACGCCGTTTCCGTTCGGGCCGTTTATCGCCGG
AGCCGGCCTGATCGTTCTGTTCTTCGGCGCGGATCTCCTGCCATTGGCGCTGAGCATTCACTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P2H7A4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.158

96.864

0.505
  pilD Vibrio campbellii strain DS40M4

52.381

95.122

0.498
  pilD Neisseria gonorrhoeae MS11

50.735

94.774

0.481
  pilD Acinetobacter baumannii D1279779

47.059

94.774

0.446
  pilD Acinetobacter nosocomialis M2

46.691

94.774

0.443

Multiple sequence alignment