Detailed information NTG0387201

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FOB35_RS27020
• Genome accession: NZ_CP044050
• Coordinates: 5316757..5317557 (-)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain FDAARGOS_627
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5311757..5322557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOB35_RS27000 (FOB35_27005)	dksA	5312338..5312793 (-)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-
FOB35_RS27005 (FOB35_27010)	sfsA	5312956..5313672 (-)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
FOB35_RS27010 (FOB35_27015)	thpR	5313672..5314208 (-)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
FOB35_RS27015 (FOB35_27020)	hrpB	5314281..5316710 (+)	2430	WP_110215630.1	ATP-dependent helicase HrpB	-
FOB35_RS27020 (FOB35_27025)	pilD	5316757..5317557 (-)	801	WP_008807384.1	prepilin peptidase	Machinery gene
FOB35_RS27025 (FOB35_27030)	-	5317554..5318300 (-)	747	WP_023322077.1	type II secretion system protein N	-
FOB35_RS27030 (FOB35_27035)	-	5318290..5318775 (-)	486	WP_101856512.1	type II secretion system protein M	-
FOB35_RS27035 (FOB35_27040)	gspL	5318768..5319964 (-)	1197	WP_012542825.1	type II secretion system protein GspL	-
FOB35_RS27040 (FOB35_27045)	gspK	5319957..5320937 (-)	981	WP_150342356.1	type II secretion system minor pseudopilin GspK	-
FOB35_RS27045 (FOB35_27050)	gspJ	5320934..5321530 (-)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
FOB35_RS27050 (FOB35_27055)	gspI	5321527..5321892 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
FOB35_RS27055 (FOB35_27060)	gspH	5321889..5322404 (-)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=387201 FOB35_RS27020 WP_008807384.1 5316757..5317557(-) (pilD) [Klebsiella variicola strain FDAARGOS_627]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=387201 FOB35_RS27020 WP_008807384.1 5316757..5317557(-) (pilD) [Klebsiella variicola strain FDAARGOS_627]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425