Detailed information NTG0386629

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F2X40_RS10645
• Genome accession: NZ_CP043932
• Coordinates: 2137038..2137838 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 555
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2132038..2142838

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F2X40_RS10610 (F2X40_10610)	gspH	2132194..2132709 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
F2X40_RS10615 (F2X40_10615)	gspI	2132706..2133071 (+)	366	WP_048993360.1	type II secretion system minor pseudopilin GspI	-
F2X40_RS10620 (F2X40_10620)	gspJ	2133068..2133664 (+)	597	WP_064152192.1	type II secretion system minor pseudopilin GspJ	-
F2X40_RS10625 (F2X40_10625)	gspK	2133661..2134647 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
F2X40_RS10630 (F2X40_10630)	gspL	2134634..2135827 (+)	1194	WP_025985182.1	type II secretion system protein GspL	-
F2X40_RS10635 (F2X40_10635)	-	2135820..2136305 (+)	486	WP_025368434.1	type II secretion system protein M	-
F2X40_RS10640 (F2X40_10640)	-	2136295..2137041 (+)	747	WP_064152193.1	type II secretion system protein N	-
F2X40_RS10645 (F2X40_10645)	pilD	2137038..2137838 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
F2X40_RS10650 (F2X40_10650)	hrpB	2137886..2140315 (-)	2430	WP_004222692.1	ATP-dependent helicase HrpB	-
F2X40_RS10655 (F2X40_10655)	thpR	2140388..2140924 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
F2X40_RS10660 (F2X40_10660)	sfsA	2140924..2141640 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
F2X40_RS10665 (F2X40_10665)	dksA	2141803..2142258 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=386629 F2X40_RS10645 WP_004145898.1 2137038..2137838(+) (pilD) [Klebsiella pneumoniae strain 555]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=386629 F2X40_RS10645 WP_004145898.1 2137038..2137838(+) (pilD) [Klebsiella pneumoniae strain 555]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGGCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425