Detailed information NTG0386576

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F2980_RS23620
• Genome accession: NZ_CP043928
• Coordinates: 4236724..4237524 (+)
• Length: 266 a.a.
• NCBI ID: WP_065882747.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain M17277
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4231724..4242524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F2980_RS23585 (F2980_23590)	gspH	4231877..4232392 (+)	516	WP_065882744.1	type II secretion system minor pseudopilin GspH	-
F2980_RS23590 (F2980_23595)	gspI	4232389..4232754 (+)	366	WP_032452553.1	type II secretion system minor pseudopilin GspI	-
F2980_RS23595 (F2980_23600)	gspJ	4232751..4233347 (+)	597	WP_023292022.1	type II secretion system minor pseudopilin GspJ	-
F2980_RS23600 (F2980_23605)	gspK	4233344..4234324 (+)	981	WP_077269661.1	type II secretion system minor pseudopilin GspK	-
F2980_RS23605 (F2980_23610)	gspL	4234317..4235513 (+)	1197	WP_065882745.1	type II secretion system protein GspL	-
F2980_RS23610 (F2980_23615)	-	4235506..4235991 (+)	486	WP_080901681.1	type II secretion system protein M	-
F2980_RS23615 (F2980_23620)	-	4235981..4236709 (+)	729	WP_049014316.1	type II secretion system protein N	-
F2980_RS23620 (F2980_23625)	pilD	4236724..4237524 (+)	801	WP_065882747.1	prepilin peptidase	Machinery gene
F2980_RS23625 (F2980_23630)	hrpB	4237572..4240001 (-)	2430	WP_065882748.1	ATP-dependent helicase HrpB	-
F2980_RS23630 (F2980_23635)	thpR	4240074..4240610 (+)	537	WP_023292015.1	RNA 2',3'-cyclic phosphodiesterase	-
F2980_RS23635 (F2980_23640)	sfsA	4240610..4241326 (+)	717	WP_023292014.1	DNA/RNA nuclease SfsA	-
F2980_RS23640 (F2980_23645)	dksA	4241489..4241944 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29246.76 Da
• Isoelectric Point: 8.6099

>NTDB_id=386576 F2980_RS23620 WP_065882747.1 4236724..4237524(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain M17277]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDEPITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELATGLLFILAGALLAPGLALAGGLVLLSFLLILARIDAQTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLLASASGLAWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=386576 F2980_RS23620 WP_065882747.1 4236724..4237524(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain M17277]
ATGACGACATTAGCCGCTTTATCGCTGCATTTTCCCCTTGTCTGGTATGGTTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGACCCAAACCGCGGACGACGAGCCGATAACCCTCAGCA
CGCCGGGCTCATCCTGCCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTCCTGCTGATACTGGCGC
GGATTGACGCCCAGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCCCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCAGCGGGCTGGCGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425