Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   F1539_RS05040 Genome accession   NZ_CP043772
Coordinates   962037..962576 (-) Length   179 a.a.
NCBI ID   WP_005686810.1    Uniprot ID   -
Organism   Haemophilus influenzae biotype aegyptius strain HE24/F3037     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 957037..967576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1539_RS05010 (F1539_05015) fdx 957440..957781 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  F1539_RS05015 (F1539_05020) iscX 957781..957975 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  F1539_RS05020 (F1539_05025) - 958026..958640 (-) 615 WP_013525574.1 YfgM family protein -
  F1539_RS05025 (F1539_05030) hisS 958658..959929 (-) 1272 WP_013525573.1 histidine--tRNA ligase -
  F1539_RS05030 (F1539_05035) ispG 959939..961045 (-) 1107 WP_005654847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  F1539_RS05035 (F1539_05040) - 961054..961965 (-) 912 WP_013525572.1 RodZ family helix-turn-helix domain-containing protein -
  F1539_RS05040 (F1539_05045) pilF/pilF2 962037..962576 (-) 540 WP_005686810.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  F1539_RS05045 (F1539_05050) - 962677..963828 (-) 1152 WP_013525571.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  F1539_RS05050 (F1539_05055) - 964075..964953 (+) 879 WP_013525570.1 D-alanyl-D-alanine carboxypeptidase family protein -
  F1539_RS05055 (F1539_05060) - 965314..966195 (+) 882 WP_013525569.1 metal ABC transporter substrate-binding protein -
  F1539_RS05060 (F1539_05065) - 966195..967115 (+) 921 WP_013525568.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20595.25 Da        Isoelectric Point: 8.6216

>NTDB_id=385834 F1539_RS05040 WP_005686810.1 962037..962576(-) (pilF/pilF2) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=385834 F1539_RS05040 WP_005686810.1 962037..962576(-) (pilF/pilF2) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCGGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment