Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   HHM37_RS03175 Genome accession   NZ_CP051609
Coordinates   627843..629675 (-) Length   610 a.a.
NCBI ID   WP_001283421.1    Uniprot ID   P0A8G1
Organism   Escherichia coli O25b:H4-ST131 strain 2019_APHA     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 622843..634675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM37_RS03135 (HHM37_03135) - 624445..625014 (+) 570 WP_000100753.1 siphovirus Gp157 family protein -
  HHM37_RS03140 (HHM37_03140) - 625014..625196 (+) 183 WP_000916334.1 hypothetical protein -
  HHM37_RS03145 (HHM37_03145) - 625406..625669 (+) 264 WP_000833838.1 DUF4224 domain-containing protein -
  HHM37_RS03150 (HHM37_03150) - 625638..626654 (+) 1017 WP_001531780.1 tyrosine-type recombinase/integrase -
  HHM37_RS03170 (HHM37_03170) pgsA 627238..627786 (-) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  HHM37_RS03175 (HHM37_03175) uvrC 627843..629675 (-) 1833 WP_001283421.1 excinuclease ABC subunit UvrC Machinery gene
  HHM37_RS03180 (HHM37_03180) letA 629672..630328 (-) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  HHM37_RS03185 (HHM37_03185) yecU 630624..630800 (+) 177 WP_001307856.1 protein YecU -
  HHM37_RS03190 (HHM37_03190) yecF 630787..631011 (+) 225 WP_000106474.1 DUF2594 family protein YecF -
  HHM37_RS03195 (HHM37_03195) sdiA 631079..631801 (-) 723 WP_001154255.1 transcriptional regulator SdiA -
  HHM37_RS03200 (HHM37_03200) tcyN 632031..632783 (-) 753 WP_001273000.1 L-cystine ABC transporter ATP-binding protein TcyN -
  HHM37_RS03205 (HHM37_03205) tcyL 632780..633448 (-) 669 WP_001158220.1 cystine ABC transporter permease -
  HHM37_RS03210 (HHM37_03210) dcyD 633463..634449 (-) 987 WP_001128232.1 D-cysteine desulfhydrase -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68188.03 Da        Isoelectric Point: 9.3685

>NTDB_id=385597 HHM37_RS03175 WP_001283421.1 627843..629675(-) (uvrC) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=385597 HHM37_RS03175 WP_001283421.1 627843..629675(-) (uvrC) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTATGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTACTG
GAACACAACTACATCAAACTTTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCATATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGAGCGAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATTTTCCCCATTCGCCAGTGCGAAAACAGTGTTTATCGC
AACCGCTCGCGTCCGTGTCTGCAATATCAGATAGGGCGCTGTCTGGGGCCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGTCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCGGCGCGGATTCGCGACCAAATTCAGGCGGTGCGACGTGTCACC
GAAAAACAGTTTGTTTCCAATACCGGCGACGACCTTGACGTTATTGGAGTGGCGTTCGATGCGGGTATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTGGAAACCTTCGTAGGTCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGTGGCGATAGAGCGCGTTATCTGAAACTCGCGCGTACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGGCTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGTGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCCAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACTGGCATCACGCCGGGCGATGATTACGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCTA
TCGACGACAGTAAGATCCCGGATGTGATCCTTATTGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTTTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCGCTGCTACTTGGCGTTGCCAAAGGAGCAGATCGTAAGGCTGGGCTGGA
AACGCTGTTCTTTGAGCCGGAAGGTGAGGGATTTAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACACGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGTTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGTCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A8G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374