Detailed information NTG0385411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B7D33_RS04280
• Genome accession: NZ_CP043597
• Coordinates: 889457..890257 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain WCHKP085031
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 884457..895257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7D33_RS04260 (B7D33_04275)	dksA	885037..885492 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
B7D33_RS04265 (B7D33_04280)	sfsA	885655..886371 (-)	717	WP_048289003.1	DNA/RNA nuclease SfsA	-
B7D33_RS04270 (B7D33_04285)	thpR	886371..886907 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
B7D33_RS04275 (B7D33_04290)	hrpB	886980..889409 (+)	2430	WP_094645106.1	ATP-dependent helicase HrpB	-
B7D33_RS04280 (B7D33_04295)	pilD	889457..890257 (-)	801	WP_004145898.1	A24 family peptidase	Machinery gene
B7D33_RS04285 (B7D33_04300)	-	890254..891000 (-)	747	WP_023284692.1	type II secretion system protein N	-
B7D33_RS04290 (B7D33_04305)	-	890990..891475 (-)	486	WP_004151943.1	type II secretion system protein M	-
B7D33_RS04295 (B7D33_04310)	gspL	891468..892661 (-)	1194	WP_032446429.1	type II secretion system protein GspL	-
B7D33_RS04300 (B7D33_04315)	gspK	892648..893634 (-)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
B7D33_RS04305 (B7D33_04320)	gspJ	893631..894227 (-)	597	WP_094645107.1	type II secretion system minor pseudopilin GspJ	-
B7D33_RS04310 (B7D33_04325)	gspI	894224..894589 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
B7D33_RS04315 (B7D33_04330)	gspH	894586..895101 (-)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=385411 B7D33_RS04280 WP_004145898.1 889457..890257(-) (pilD) [Klebsiella pneumoniae strain WCHKP085031]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=385411 B7D33_RS04280 WP_004145898.1 889457..890257(-) (pilD) [Klebsiella pneumoniae strain WCHKP085031]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425