Detailed information NTG0385278

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F0Q09_RS11495
• Genome accession: NZ_CP043576
• Coordinates: 2515714..2516580 (-)
• Length: 288 a.a.
• NCBI ID: WP_003247536.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain T25-27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2510714..2521580

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0Q09_RS11460	-	2511228..2512523 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
F0Q09_RS11465	-	2512608..2512781 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
F0Q09_RS11470	-	2512914..2513543 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
F0Q09_RS11475	-	2513599..2514030 (-)	432	WP_060538607.1	hypothetical protein	-
F0Q09_RS11480	-	2514117..2514824 (+)	708	WP_182375514.1	energy-coupling factor ABC transporter permease	-
F0Q09_RS11485	yacG	2514894..2515094 (-)	201	WP_003247540.1	DNA gyrase inhibitor YacG	-
F0Q09_RS11490	coaE	2515091..2515714 (-)	624	WP_003247538.1	dephospho-CoA kinase	-
F0Q09_RS11495	pilD	2515714..2516580 (-)	867	WP_003247536.1	prepilin peptidase	Machinery gene
F0Q09_RS11500	-	2516583..2517791 (-)	1209	WP_003247533.1	type II secretion system F family protein	-
F0Q09_RS11505	-	2518008..2518418 (+)	411	WP_004575718.1	pilin	-
F0Q09_RS28195	-	2518816..2518952 (+)	137	Protein_2258	Fic family protein	-
F0Q09_RS11515	-	2519092..2520492 (+)	1401	WP_003247530.1	PAAR domain-containing protein	-
F0Q09_RS11520	-	2520485..2520874 (+)	390	WP_003247529.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31808.87 Da
• Isoelectric Point: 7.9101

>NTDB_id=385278 F0Q09_RS11495 WP_003247536.1 2515714..2516580(-) (pilD) [Pseudomonas putida strain T25-27]
MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHPRFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALLALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LVGLCLLRFRRHALGTAIPFGPYLAIAGWIAVLWGDEIYASYIQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=385278 F0Q09_RS11495 WP_003247536.1 2515714..2516580(-) (pilD) [Pseudomonas putida strain T25-27]
ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACCCGCGTTTCGACCTGTGCCTGCCGGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGCTGG
CGTTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTGGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCA
CTTGTTGGCCTGTGCTTGCTGCGTTTTCGCCGGCATGCCTTGGGCACGGCTATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATACAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.986	96.181	0.5
pilD	Acinetobacter baumannii D1279779	49.11	97.569	0.479
pilD	Acinetobacter nosocomialis M2	48.399	97.569	0.472
pilD	Vibrio campbellii strain DS40M4	49.091	95.486	0.469
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451