Detailed information NTG0385207

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F0P98_RS04440
• Genome accession: NZ_CP043570
• Coordinates: 1003589..1004452 (-)
• Length: 287 a.a.
• NCBI ID: WP_054667255.1
• Uniprot ID: A0A0S1B224
• Organism: Stenotrophomonas acidaminiphila strain T25-65
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 998589..1009452

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0P98_RS04415	rimK	999159..1000034 (+)	876	WP_056931223.1	30S ribosomal protein S6--L-glutamate ligase	-
F0P98_RS04420	-	1000244..1000714 (+)	471	WP_162486451.1	hypothetical protein	-
F0P98_RS04425	-	1000881..1001558 (+)	678	WP_054667261.1	response regulator transcription factor	-
F0P98_RS04430	-	1001551..1002888 (+)	1338	WP_054667259.1	HAMP domain-containing sensor histidine kinase	-
F0P98_RS04435	coaE	1002919..1003542 (-)	624	WP_182212757.1	dephospho-CoA kinase	-
F0P98_RS04440	pilD	1003589..1004452 (-)	864	WP_054667255.1	A24 family peptidase	Machinery gene
F0P98_RS04445	-	1004460..1005719 (-)	1260	WP_054667253.1	type II secretion system F family protein	-
F0P98_RS18020	-	1006122..1006484 (+)	363	WP_182212758.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
F0P98_RS17980	-	1006614..1006949 (+)	336	WP_082394110.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
F0P98_RS04460	-	1007038..1007469 (+)	432	WP_054667248.1	GtrA family protein	-
F0P98_RS04465	-	1007511..1009010 (+)	1500	WP_198421473.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 31856.83 Da
• Isoelectric Point: 8.4746

>NTDB_id=385207 F0P98_RS04440 WP_054667255.1 1003589..1004452(-) (pilD) [Stenotrophomonas acidaminiphila strain T25-65]
MAFLDQHPGIGYPAAAGLGLLIGSFLNVVILRLPRRMEWQWKRDAREILGEPDIYEPPPPGVVVEPSHCPHCKHKLSWYE
NIPLFSWLLQRGRCRHCQAPISIQYPLVELLTAVLVLASVWQFGFGWQGFGAIVLTCFLIALSGIDLRTQLLPDQLTLPL
MWLGLIGSMDNLYMPTKPALIGAAVGYLSLWTVWWLFKQITGKEGMGHGDFKLLAALGAWCGLKGILPIILLSSLVGAIV
GSVWLYARGRDRATPIPFGPYLAIAGWLVFMWGDPMIAAYRQWAGLA

Nucleotide

• Length: 864 bp

>NTDB_id=385207 F0P98_RS04440 WP_054667255.1 1003589..1004452(-) (pilD) [Stenotrophomonas acidaminiphila strain T25-65]
ATGGCATTTCTCGACCAGCATCCCGGCATCGGCTACCCGGCCGCTGCCGGATTGGGGTTGCTGATCGGCAGCTTCCTCAA
CGTTGTGATCCTGCGCCTGCCCCGGCGCATGGAGTGGCAATGGAAGCGCGATGCGCGCGAGATCCTCGGTGAGCCGGACA
TCTACGAGCCGCCCCCGCCGGGCGTGGTGGTGGAGCCGTCGCATTGCCCGCACTGCAAGCACAAGCTGAGCTGGTACGAG
AACATCCCGCTGTTCAGCTGGCTGCTGCAGCGGGGACGCTGCCGCCATTGCCAGGCGCCGATCTCCATCCAGTACCCGCT
GGTTGAACTGCTGACCGCCGTACTGGTGCTGGCCAGCGTGTGGCAGTTCGGCTTCGGCTGGCAGGGCTTCGGTGCGATCG
TGCTGACCTGCTTCCTGATCGCACTGTCCGGCATCGACCTGCGCACCCAGCTGCTGCCCGACCAGCTGACCCTGCCACTG
ATGTGGCTGGGACTGATCGGCAGCATGGACAACCTGTACATGCCCACCAAACCTGCACTGATCGGTGCCGCGGTGGGCTA
CCTGTCGCTATGGACCGTGTGGTGGCTGTTCAAGCAGATCACCGGCAAGGAAGGCATGGGCCACGGCGATTTCAAGCTGC
TGGCGGCGCTGGGCGCCTGGTGCGGACTGAAGGGCATCCTGCCGATCATCCTGCTGTCCTCGCTGGTGGGCGCCATCGTC
GGCTCGGTCTGGCTGTACGCACGCGGACGCGACCGCGCCACGCCCATCCCGTTCGGCCCCTACCTGGCCATCGCCGGCTG
GCTGGTCTTCATGTGGGGTGACCCGATGATCGCCGCTTACCGGCAGTGGGCCGGCCTGGCGTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (133-241)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-123)

3D structure

• AlphaFold DB: A0A0S1B224

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	49.822	97.909	0.488
pilD	Acinetobacter baumannii D1279779	49.11	97.909	0.481
pilD	Vibrio cholerae strain A1552	48.921	96.864	0.474
pilD	Vibrio campbellii strain DS40M4	47.703	98.606	0.47
pilD	Neisseria gonorrhoeae MS11	45.775	98.955	0.453