Detailed information NTG0385160

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F0H50_RS20260
• Genome accession: NZ_CP043562
• Coordinates: 4096264..4097064 (+)
• Length: 266 a.a.
• NCBI ID: WP_149362563.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain P094-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4091264..4102064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0H50_RS20225	gspH	4091420..4091929 (+)	510	WP_038806668.1	type II secretion system minor pseudopilin GspH	-
F0H50_RS20230	gspI	4091932..4092297 (+)	366	WP_065804587.1	type II secretion system minor pseudopilin GspI	-
F0H50_RS20235	gspJ	4092294..4092890 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
F0H50_RS20240	gspK	4092887..4093873 (+)	987	WP_077254427.1	type II secretion system minor pseudopilin GspK	-
F0H50_RS20245	gspL	4093860..4095053 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
F0H50_RS20250	-	4095046..4095531 (+)	486	WP_004145896.1	type II secretion system protein M	-
F0H50_RS20255	-	4095521..4096267 (+)	747	WP_064152193.1	type II secretion system protein N	-
F0H50_RS20260	pilD	4096264..4097064 (+)	801	WP_149362563.1	prepilin peptidase	Machinery gene
F0H50_RS20265	hrpB	4097112..4099541 (-)	2430	WP_149362564.1	ATP-dependent helicase HrpB	-
F0H50_RS20270	thpR	4099614..4100150 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
F0H50_RS20275	sfsA	4100150..4100866 (+)	717	WP_048265185.1	DNA/RNA nuclease SfsA	-
F0H50_RS20280	dksA	4101029..4101484 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29458.12 Da
• Isoelectric Point: 9.1727

>NTDB_id=385160 F0H50_RS20260 WP_149362563.1 4096264..4097064(+) (pilD) [Klebsiella pneumoniae strain P094-1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWRYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=385160 F0H50_RS20260 WP_149362563.1 4096264..4097064(+) (pilD) [Klebsiella pneumoniae strain P094-1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGCGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.811	99.624	0.496
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.678	98.12	0.429