Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EVS87_RS11515 Genome accession   NZ_CP043559
Coordinates   2227756..2228826 (-) Length   356 a.a.
NCBI ID   WP_035390817.1    Uniprot ID   -
Organism   Bacillus altitudinis strain CHB19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2222756..2233826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVS87_RS11465 (EVS87_011465) sipW 2222851..2223423 (-) 573 WP_017357979.1 signal peptidase I SipW -
  EVS87_RS11470 (EVS87_011470) tapA 2223473..2224000 (-) 528 WP_135009242.1 amyloid fiber anchoring/assembly protein TapA -
  EVS87_RS11475 (EVS87_011475) - 2224253..2224579 (+) 327 WP_058336653.1 DUF3889 domain-containing protein -
  EVS87_RS11480 (EVS87_011480) - 2224614..2224808 (-) 195 WP_135009243.1 YqzE family protein -
  EVS87_RS11485 (EVS87_011485) - 2224869..2225252 (-) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  EVS87_RS11490 (EVS87_011490) comGF 2225249..2225746 (-) 498 WP_034647459.1 competence type IV pilus minor pilin ComGF -
  EVS87_RS11495 (EVS87_011495) comGE 2225688..2226002 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  EVS87_RS11500 (EVS87_011500) comGD 2225986..2226432 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  EVS87_RS11505 (EVS87_011505) comGC 2226425..2226718 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  EVS87_RS11510 (EVS87_011510) comGB 2226735..2227775 (-) 1041 WP_058336649.1 competence type IV pilus assembly protein ComGB -
  EVS87_RS11515 (EVS87_011515) comGA 2227756..2228826 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  EVS87_RS11520 (EVS87_011520) - 2229284..2230156 (-) 873 WP_041506953.1 STAS domain-containing protein -
  EVS87_RS11530 (EVS87_011530) - 2230382..2230762 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  EVS87_RS11535 (EVS87_011535) - 2230975..2231217 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  EVS87_RS11540 (EVS87_011540) - 2231266..2231904 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  EVS87_RS11545 (EVS87_011545) - 2232065..2232238 (+) 174 WP_135009244.1 DUF2759 domain-containing protein -
  EVS87_RS11550 (EVS87_011550) - 2232263..2232577 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40396.75 Da        Isoelectric Point: 9.0449

>NTDB_id=385117 EVS87_RS11515 WP_035390817.1 2227756..2228826(-) (comGA) [Bacillus altitudinis strain CHB19]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=385117 EVS87_RS11515 WP_035390817.1 2227756..2228826(-) (comGA) [Bacillus altitudinis strain CHB19]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGTTCATTGGCT
GTGATGTTAAGGCGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAGGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646

Multiple sequence alignment