Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FZF10_RS13600 Genome accession   NZ_CP043440
Coordinates   2519789..2520796 (+) Length   335 a.a.
NCBI ID   WP_149137595.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain MJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2514789..2525796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZF10_RS13575 coaD 2515097..2515581 (-) 485 Protein_2294 pantetheine-phosphate adenylyltransferase -
  FZF10_RS13580 rsmD 2515774..2516418 (-) 645 WP_187645087.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FZF10_RS13585 - 2516514..2517881 (-) 1368 WP_149136785.1 pitrilysin family protein -
  FZF10_RS13590 - 2517891..2519441 (-) 1551 WP_149136786.1 pitrilysin family protein -
  FZF10_RS13600 pilA 2519789..2520796 (+) 1008 WP_149137595.1 signal recognition particle-docking protein FtsY Machinery gene
  FZF10_RS13605 - 2521032..2523365 (+) 2334 WP_149136787.1 carboxypeptidase regulatory-like domain-containing protein -
  FZF10_RS13610 maiA 2523460..2524104 (-) 645 WP_187645187.1 maleylacetoacetate isomerase -
  FZF10_RS13615 - 2524112..2524813 (-) 702 WP_149136789.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36145.90 Da        Isoelectric Point: 5.8223

>NTDB_id=384106 FZF10_RS13600 WP_149137595.1 2519789..2520796(+) (pilA) [Cupriavidus campinensis strain MJ1]
MPVPAQVPPPPPAPVAYEPPVVEELELTPPPAQTAEAKRGWMTRLRAGLSKTSRNLGTLFVGVKVDEALFEELETALLMA
DAGVEATEFLLGELRRRVKNERLETAEGVKTALRELLTDLLRPLEKTMELGREQPLVMMIAGVNGAGKTTSIGKLCKHFQ
AYHQSVLLAAGDTFRAAAREQLTIWGERNNVTVVAQEGGDPAAVIFDAVNAARARKIDIVMADTAGRLPTQLHLMEELKK
VKRVIGKSMPTAPHEVLLVIDANTGQNALSQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVED
LQPFKAEEFAEALLG

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=384106 FZF10_RS13600 WP_149137595.1 2519789..2520796(+) (pilA) [Cupriavidus campinensis strain MJ1]
ATCCCCGTTCCCGCCCAGGTGCCGCCTCCGCCGCCGGCGCCGGTCGCCTATGAGCCGCCGGTTGTCGAGGAACTCGAACT
GACGCCGCCGCCGGCCCAGACCGCCGAGGCCAAGCGCGGCTGGATGACGCGCCTGCGTGCCGGGCTGTCGAAGACGAGCC
GGAACCTCGGCACGCTGTTCGTCGGCGTGAAGGTGGACGAGGCGCTGTTCGAGGAACTCGAAACCGCGCTGCTGATGGCC
GACGCCGGCGTGGAAGCCACCGAATTCCTGCTCGGCGAACTCCGCCGCCGCGTGAAGAACGAGCGGCTCGAGACCGCCGA
GGGCGTGAAGACCGCCCTGCGCGAGTTGCTGACCGACCTGCTGCGCCCGCTTGAGAAGACCATGGAGCTGGGCCGCGAGC
AACCGCTGGTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTCTGCAAGCACTTCCAG
GCCTATCACCAGTCGGTGCTGCTGGCGGCCGGTGACACCTTCCGCGCCGCCGCACGCGAGCAACTGACGATCTGGGGCGA
GCGCAACAACGTCACCGTGGTGGCTCAGGAAGGCGGCGACCCGGCGGCGGTGATCTTCGATGCCGTGAACGCGGCGCGCG
CGCGCAAGATCGACATCGTGATGGCGGACACCGCGGGGCGCCTGCCCACGCAGCTGCATCTGATGGAAGAACTGAAGAAG
GTGAAGCGCGTGATCGGCAAGTCGATGCCGACGGCCCCGCACGAGGTGCTGCTGGTGATCGATGCCAACACCGGGCAGAA
CGCCCTGTCCCAGACCCGCGCCTTCGATGACGCGCTGGGCCTGACCGGCCTGATCGTGACCAAGCTGGACGGCACGGCCA
AGGGCGGCATCCTCGCCGCCATCGCCCGGCAACGCCCGGTGCCGGTGTACTTCATCGGCGTGGGCGAGAAGGTGGAAGAT
CTTCAGCCGTTCAAGGCGGAAGAATTCGCTGAAGCGCTACTCGGGTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(59-115)

SRP54-type protein, GTPase domain (SRP54)

(136-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

62.662

91.94

0.576

Multiple sequence alignment