Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CYAN7822_RS08800 Genome accession   NC_014501
Coordinates   1983457..1985487 (-) Length   676 a.a.
NCBI ID   WP_013321897.1    Uniprot ID   E0U9I9
Organism   Gloeothece verrucosa PCC 7822     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1978457..1990487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYAN7822_RS08785 (Cyan7822_1801) raiA 1979616..1980215 (+) 600 WP_013321894.1 ribosome-associated translation inhibitor RaiA -
  CYAN7822_RS08790 (Cyan7822_1802) - 1980888..1982102 (-) 1215 WP_013321895.1 type II secretion system F family protein -
  CYAN7822_RS08795 (Cyan7822_1803) pilT 1982238..1983356 (-) 1119 WP_173362903.1 type IV pilus twitching motility protein PilT Machinery gene
  CYAN7822_RS08800 (Cyan7822_1804) pilB/pilB1 1983457..1985487 (-) 2031 WP_013321897.1 GspE/PulE family protein Machinery gene
  CYAN7822_RS08805 (Cyan7822_1805) grpE 1985728..1986591 (+) 864 WP_013321898.1 nucleotide exchange factor GrpE -
  CYAN7822_RS08810 (Cyan7822_1806) dnaK 1987074..1989251 (+) 2178 WP_013321899.1 molecular chaperone DnaK -
  CYAN7822_RS08815 (Cyan7822_1807) - 1989389..1989826 (+) 438 WP_013321900.1 hypothetical protein -
  CYAN7822_RS08820 (Cyan7822_1808) - 1989954..1990124 (-) 171 WP_245602713.1 hypothetical protein -

Sequence

Protein


Download         Length: 676 a.a.        Molecular weight: 75144.08 Da        Isoelectric Point: 5.6651

>NTDB_id=38380 CYAN7822_RS08800 WP_013321897.1 1983457..1985487(-) (pilB/pilB1) [Gloeothece verrucosa PCC 7822]
MTYSTSSKKSRALIKSGVSQLTPIGNKLVQAGYVGPDQMQQALIETRKSGRTLVEVLQALTGRQMPPDLQRSYKKHQLFE
LKVVYGVDCIDPEISQFDEAEMARLVDTLIPIDVCRRYKIIPLKKIETEPVGILVAMVDPDNLDAIDDVNRILRPRNLTL
HRQVITQQDYEQLLEKFHDAQSVLEEEKQRLAKEKELEKLGNLTDIVDSIDLGAVQDVGEDATDDLGADDAGKAPVINLV
NKILAKALQEGTSDIHVEPQEEHLRVRFRKDGVLHQAFDPLPRQITPAVIARFKIMADLDIAERRLPQDGKIRRMFQGRK
VDFRVNTLPSRYGEKVCLRILDNSATQLGLDKLITDQRTLQIVRELASRPFGLLLVTGPTGSGKSTSLYSVLAERNHPGV
NISTAEDPIEYSLPGITQVQVIREKGMDFSSILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGA
IARLDEMGIEPFMVSGALLGVLAQRLMRRVCSECRVAYNPSKAELARFGLSASNEDEVTFYKANTLTSDELAEAKQQGSV
CAKCGGKGYKGRVGVYEVMQNSERLQKLINEGATTDRIKDAAVEEGMTTLLAYSLNLVREGYTTLEEVERVTFTDSGLEA
ELKAKRKSGLTCRTCKAELVPEWLDCPYCMTARFSD

Nucleotide


Download         Length: 2031 bp        

>NTDB_id=38380 CYAN7822_RS08800 WP_013321897.1 1983457..1985487(-) (pilB/pilB1) [Gloeothece verrucosa PCC 7822]
ATGACTTATTCTACATCCTCCAAGAAAAGCCGAGCGCTAATCAAAAGTGGGGTTTCTCAGTTGACCCCCATCGGGAACAA
GCTGGTTCAAGCTGGCTATGTTGGCCCAGACCAAATGCAGCAAGCGTTGATTGAAACCCGTAAGTCAGGCCGCACCCTCG
TTGAGGTTCTACAAGCGCTTACAGGACGACAGATGCCTCCTGACTTGCAGCGTTCTTACAAAAAACATCAACTGTTTGAG
CTTAAGGTGGTCTACGGCGTTGACTGTATTGATCCAGAAATTAGCCAGTTCGATGAAGCCGAAATGGCAAGACTGGTAGA
TACTTTAATCCCCATTGATGTTTGTCGGCGCTATAAGATAATTCCTTTAAAAAAAATTGAAACTGAACCTGTTGGCATCT
TAGTGGCTATGGTCGATCCAGATAATTTGGATGCCATTGATGATGTTAATCGCATTTTACGTCCTCGCAATTTAACTTTA
CACCGTCAGGTCATTACCCAACAAGATTATGAGCAATTGCTGGAAAAATTCCATGATGCTCAGTCTGTACTTGAAGAAGA
GAAACAACGTTTAGCTAAAGAGAAAGAGCTTGAAAAATTAGGAAATTTAACTGATATTGTTGACAGTATTGATCTGGGTG
CTGTTCAAGATGTTGGCGAAGATGCCACCGATGATCTAGGAGCCGATGATGCGGGTAAAGCTCCTGTGATTAATTTAGTC
AATAAAATTCTTGCTAAAGCTCTCCAAGAAGGCACTTCCGACATTCACGTTGAACCTCAAGAAGAACATTTACGGGTTCG
TTTTCGTAAGGACGGGGTACTTCATCAAGCGTTTGACCCCCTGCCGCGACAAATTACGCCCGCCGTGATTGCTCGTTTTA
AAATCATGGCCGATTTAGATATTGCTGAAAGACGACTGCCTCAAGATGGTAAGATTCGCCGTATGTTCCAAGGTCGTAAA
GTGGATTTTCGGGTTAATACTTTACCGAGTCGCTATGGGGAGAAAGTCTGTTTACGGATTTTAGATAACTCAGCCACGCA
GTTAGGGTTAGATAAGCTCATTACTGACCAAAGAACTTTACAAATTGTCAGAGAGCTTGCTAGTCGTCCTTTTGGTTTAC
TTTTGGTAACGGGGCCCACTGGTTCGGGTAAGTCGACCAGTTTGTATTCGGTTTTAGCTGAACGCAATCACCCTGGGGTT
AATATCAGCACCGCAGAAGACCCGATTGAGTATTCTTTACCCGGCATCACCCAAGTTCAGGTTATTCGCGAAAAAGGGAT
GGATTTTTCTTCGATTCTGCGGGCTTTTATGCGTCAAGACCCTGATGTGATCCTTGTGGGAGAAACTCGCGACAAAGAAA
CGGCTAAAACGGCAATTGAAGCGGCTTTAACCGGTCACCTGGTTTTAACGACGCTTCATACCAATGATGCGGCTGGCGCG
ATCGCCCGTCTTGATGAAATGGGAATCGAACCTTTTATGGTTTCTGGGGCGTTACTTGGGGTTTTGGCTCAACGCTTAAT
GCGGCGAGTCTGCTCAGAGTGCCGTGTAGCCTACAACCCGAGCAAAGCGGAATTGGCTCGCTTTGGTTTATCGGCCTCTA
ATGAAGACGAAGTGACCTTCTACAAAGCCAATACTTTAACATCTGATGAACTGGCTGAAGCTAAACAGCAAGGCTCTGTT
TGTGCTAAATGTGGCGGGAAAGGTTACAAGGGACGAGTTGGGGTTTATGAAGTCATGCAGAACAGTGAGCGGCTACAAAA
ATTAATTAATGAAGGAGCCACCACTGATCGGATTAAAGACGCGGCTGTAGAAGAGGGAATGACCACTCTATTAGCTTATA
GCTTGAATCTGGTACGAGAAGGTTACACCACTTTAGAAGAAGTCGAACGGGTAACTTTTACCGATTCGGGTTTAGAAGCT
GAACTGAAGGCTAAACGGAAAAGCGGACTCACTTGCCGCACTTGTAAGGCTGAATTAGTTCCTGAATGGCTCGATTGTCC
TTATTGTATGACTGCTCGTTTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(242-509)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U9I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.977

96.893

0.707

Multiple sequence alignment