Detailed information NTG0383695

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FZE25_RS12410
• Genome accession: NZ_CP043416
• Coordinates: 2525467..2526081 (-)
• Length: 204 a.a.
• NCBI ID: WP_020956097.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain ONU 553
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2520467..2531081

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FZE25_RS12380 (FZE25_12175)	rpsT	2520770..2521036 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
FZE25_RS12385 (FZE25_12180)	holA	2521053..2522094 (-)	1042	Protein_2378	DNA polymerase III subunit delta	-
FZE25_RS12390 (FZE25_12185)	-	2522129..2522285 (-)	157	Protein_2379	hypothetical protein	-
FZE25_RS12395 (FZE25_12190)	-	2522326..2522460 (+)	135	WP_003152870.1	YqzM family protein	-
FZE25_RS12400 (FZE25_12195)	comEC	2522479..2524830 (-)	2352	WP_149061751.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FZE25_RS12405 (FZE25_12200)	-	2524831..2525400 (-)	570	WP_007408264.1	ComE operon protein 2	-
FZE25_RS12410 (FZE25_12205)	comEA	2525467..2526081 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
FZE25_RS12415 (FZE25_12210)	comER	2526140..2526961 (+)	822	WP_020956098.1	late competence protein ComER	-
FZE25_RS12420 (FZE25_12215)	-	2527031..2527768 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
FZE25_RS12425 (FZE25_12220)	rsfS	2527765..2528121 (-)	357	WP_007408260.1	ribosome silencing factor	-
FZE25_RS12430 (FZE25_12225)	yqeK	2528139..2528699 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
FZE25_RS12435 (FZE25_12230)	-	2528689..2529258 (-)	570	WP_042635364.1	nicotinate-nucleotide adenylyltransferase	-
FZE25_RS12440 (FZE25_12235)	yhbY	2529269..2529559 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
FZE25_RS12445 (FZE25_12240)	aroE	2529553..2530395 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22251.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=383695 FZE25_RS12410 WP_020956097.1 2525467..2526081(-) (comEA) [Bacillus velezensis strain ONU 553]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=383695 FZE25_RS12410 WP_020956097.1 2525467..2526081(-) (comEA) [Bacillus velezensis strain ONU 553]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363