Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FX981_RS18045 Genome accession   NZ_CP043404
Coordinates   3550866..3551246 (-) Length   126 a.a.
NCBI ID   WP_065214465.1    Uniprot ID   -
Organism   Bacillus safensis strain PgKB20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3545866..3556246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX981_RS18010 (FX981_03619) - 3546453..3546683 (-) 231 WP_024425132.1 DUF896 domain-containing protein -
  FX981_RS18015 (FX981_03620) - 3546753..3547400 (-) 648 WP_025093247.1 YneB family resolvase-like protein -
  FX981_RS18020 (FX981_03621) yneA 3547418..3547729 (-) 312 WP_024425130.1 cell division suppressor protein YneA -
  FX981_RS18025 (FX981_03622) lexA 3547888..3548508 (+) 621 WP_024425129.1 transcriptional repressor LexA -
  FX981_RS18030 - 3548554..3549000 (-) 447 WP_149126619.1 hypothetical protein -
  FX981_RS18035 (FX981_03623) - 3549297..3549839 (+) 543 WP_024425127.1 IseA DL-endopeptidase inhibitor family protein -
  FX981_RS18040 (FX981_03624) - 3549872..3550720 (-) 849 WP_024427077.1 STAS domain-containing protein -
  FX981_RS18045 (FX981_03625) nucA/comI 3550866..3551246 (-) 381 WP_065214465.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FX981_RS18050 (FX981_03626) - 3551504..3552412 (-) 909 WP_024425124.1 trypsin-like serine peptidase -
  FX981_RS18055 (FX981_03627) - 3552606..3555749 (-) 3144 WP_149126620.1 bifunctional cytochrome P450/NADPH--P450 reductase -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13833.49 Da        Isoelectric Point: 7.9051

>NTDB_id=383532 FX981_RS18045 WP_065214465.1 3550866..3551246(-) (nucA/comI) [Bacillus safensis strain PgKB20]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISEFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=383532 FX981_RS18045 WP_065214465.1 3550866..3551246(-) (nucA/comI) [Bacillus safensis strain PgKB20]
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGAGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment