Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FX981_RS15315 Genome accession   NZ_CP043404
Coordinates   3032529..3033599 (+) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus safensis strain PgKB20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3027529..3038599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX981_RS15280 (FX981_03071) - 3028857..3029171 (+) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  FX981_RS15285 (FX981_03072) - 3029396..3029569 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  FX981_RS15290 (FX981_03073) - 3029727..3030365 (+) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  FX981_RS15295 (FX981_03074) - 3030413..3030655 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  FX981_RS15300 (FX981_03075) - 3030869..3031249 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FX981_RS15310 (FX981_03077) - 3031473..3032348 (+) 876 WP_024422979.1 STAS domain-containing protein -
  FX981_RS15315 (FX981_03078) comGA 3032529..3033599 (+) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  FX981_RS15320 (FX981_03079) comGB 3033580..3034620 (+) 1041 WP_103131948.1 competence type IV pilus assembly protein ComGB -
  FX981_RS15325 (FX981_03080) comGC 3034637..3034930 (+) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  FX981_RS15330 (FX981_03081) comGD 3034923..3035369 (+) 447 WP_149126476.1 competence type IV pilus minor pilin ComGD -
  FX981_RS19605 (FX981_03082) comGE 3035353..3035667 (+) 315 WP_149126477.1 competence type IV pilus minor pilin ComGE -
  FX981_RS15340 (FX981_03083) comGF 3035654..3036106 (+) 453 WP_187470543.1 competence type IV pilus minor pilin ComGF -
  FX981_RS15345 (FX981_03084) - 3036103..3036486 (+) 384 WP_073206276.1 competence type IV pilus minor pilin ComGG -
  FX981_RS15350 (FX981_03085) - 3036546..3036740 (+) 195 WP_149126478.1 YqzE family protein -
  FX981_RS15355 (FX981_03086) - 3036775..3037053 (-) 279 WP_171464609.1 DUF3889 domain-containing protein -
  FX981_RS15360 (FX981_03087) tapA 3037353..3037880 (+) 528 WP_024422972.1 amyloid fiber anchoring/assembly protein TapA -
  FX981_RS15365 (FX981_03088) sipW 3037933..3038505 (+) 573 WP_024422971.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=383526 FX981_RS15315 WP_025092972.1 3032529..3033599(+) (comGA) [Bacillus safensis strain PgKB20]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=383526 FX981_RS15315 WP_025092972.1 3032529..3033599(+) (comGA) [Bacillus safensis strain PgKB20]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACTTTGCCGACAGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAACGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment