Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HF908_RS01710 Genome accession   NZ_CP051171
Coordinates   366569..368125 (-) Length   518 a.a.
NCBI ID   WP_193027246.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361569..373125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS01695 (HF908_01715) - 362095..363378 (-) 1284 WP_193027240.1 hypothetical protein -
  HF908_RS01700 (HF908_01720) gspF 363676..364887 (-) 1212 WP_193027242.1 type II secretion system inner membrane protein GspF -
  HF908_RS01705 (HF908_01725) - 364904..366472 (-) 1569 WP_193027244.1 DUF4153 domain-containing protein -
  HF908_RS01710 (HF908_01730) pilB 366569..368125 (-) 1557 WP_193027246.1 type II secretion system ATPase GspE Machinery gene
  HF908_RS01715 (HF908_01735) gspD 368160..370577 (-) 2418 WP_193027248.1 type II secretion system secretin GspD -
  HF908_RS01720 (HF908_01740) - 370611..371462 (-) 852 WP_193027251.1 type II secretion system protein N -
  HF908_RS01725 (HF908_01745) gspM 371466..372044 (-) 579 WP_193027253.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55781.77 Da        Isoelectric Point: 5.3667

>NTDB_id=382990 HF908_RS01710 WP_193027246.1 366569..368125(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2279]
MATQASSTDARTLETPSPSAVRLVPYVFARDAKLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSTA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDHLINQPHGIVLVTGPTGSGKTTTLYAALSRLDAGSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGVAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAAES
EIKALAIGQGMQTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=382990 HF908_RS01710 WP_193027246.1 366569..368125(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2279]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGACGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTACGT
GTTCGCGCGCGACGCCAAGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAACTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGATGCCGCCGCGCTTTCCACCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTGGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGATCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCGCGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAAGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGGCCGGTGGACGTGC
GCGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTACTGCGTCTGCTCGACAAGGAAGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCATCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACTGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCGGCAGCACCAACATCATGA
CGGTCGAGGACCCGATCGAGTACGACCTGGACGGCATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGATCCGGACGTGGTGATGATCGGCGAAATCCGCGACCTGGAGACCGCGCAGAT
CGCAGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAACTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCTGA
CGGCGTTGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGTTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGGCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCAGACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGATTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

45.033

87.452

0.394
  pilB Vibrio cholerae strain A1552

45.434

86.68

0.394
  pilB Vibrio parahaemolyticus RIMD 2210633

44.273

87.645

0.388
  pilF Thermus thermophilus HB27

46.539

80.888

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373