Detailed information NTG0382929

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HF909_RS03165
• Genome accession: NZ_CP051169
• Coordinates: 657481..658362 (-)
• Length: 293 a.a.
• NCBI ID: WP_282107753.1
• Uniprot ID: -
• Organism: Ralstonia pseudosolanacearum strain RUN2474
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 652481..663362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF909_RS03135 (HF909_03165)	argJ	653191..654420 (+)	1230	WP_193035773.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
HF909_RS03140 (HF909_03170)	-	654436..655320 (+)	885	WP_193035775.1	ATP-binding protein	-
HF909_RS03145 (HF909_03175)	-	655298..655759 (+)	462	WP_193035777.1	NUDIX domain-containing protein	-
HF909_RS03150 (HF909_03180)	yacG	655752..655967 (-)	216	WP_011002736.1	DNA gyrase inhibitor YacG	-
HF909_RS03155 (HF909_03185)	zapD	655978..656736 (-)	759	WP_020747465.1	cell division protein ZapD	-
HF909_RS03160 (HF909_03190)	coaE	656854..657468 (-)	615	WP_193027553.1	dephospho-CoA kinase	-
HF909_RS03165 (HF909_03195)	pilD	657481..658362 (-)	882	WP_282107753.1	prepilin peptidase	Machinery gene
HF909_RS03170 (HF909_03200)	pilC	658365..659630 (-)	1266	WP_193035781.1	type II secretion system F family protein	Machinery gene
HF909_RS03175 (HF909_03205)	pilB	659675..661402 (-)	1728	WP_193027556.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HF909_RS03180 (HF909_03210)	-	661650..662927 (-)	1278	WP_193027557.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31609.59 Da
• Isoelectric Point: 8.3415

>NTDB_id=382929 HF909_RS03165 WP_282107753.1 657481..658362(-) (pilD) [Ralstonia pseudosolanacearum strain RUN2474]
MPGMFVIPTLAQLPAWFVIGAGTLLGLLIGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHSEPYNLVVPRSACPSCGH
QIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFGMFARLPDAVIGATAGYLFLWSIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLSS
VVGLVFGVVRIARGATSESPFSFGPFIAGAGVISLLAGPQLVALMGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=382929 HF909_RS03165 WP_282107753.1 657481..658362(-) (pilD) [Ralstonia pseudosolanacearum strain RUN2474]
ATGCCCGGAATGTTCGTCATTCCCACGCTGGCGCAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGGCTT
GCTGATCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCAACGAACCGCTGCCGCATTCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCATGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTGCGCGGCCGCTGCAGCGCCTGCAAGACCCCCAT
CTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACTGGCGTGCTGACCGGCGCCTGCCTGTGGCACTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATTGCCGGCTCGATGATCGACGCCGACACGCAGCTCCTGCCCGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCGGCATGTTCGCACGGCTGCCGGACGCGGTGAT
CGGCGCGACGGCGGGGTATCTATTCCTGTGGAGCATCTACTGGGCCTATAAGCTCCTGCGCGGCCGCGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGGCGCACTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGCCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTGATCTCACTGCTGGCCGGCCCGCAATTGGTGGCGCTGATGGGGCTCGCACCACTGCTGGCGCGGT
AA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.075	90.444	0.471
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	45.865	90.785	0.416
pilD	Acinetobacter baumannii D1279779	45.865	90.785	0.416