Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HF909_RS01690 Genome accession   NZ_CP051169
Coordinates   359093..360649 (-) Length   518 a.a.
NCBI ID   WP_193035660.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354093..365649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS01675 (HF909_01690) - 354619..355902 (-) 1284 WP_193027240.1 hypothetical protein -
  HF909_RS01680 (HF909_01695) gspF 356200..357411 (-) 1212 WP_193027242.1 type II secretion system inner membrane protein GspF -
  HF909_RS01685 (HF909_01700) - 357428..358996 (-) 1569 WP_193027244.1 DUF4153 domain-containing protein -
  HF909_RS01690 (HF909_01705) pilB 359093..360649 (-) 1557 WP_193035660.1 type II secretion system ATPase GspE Machinery gene
  HF909_RS01695 (HF909_01710) gspD 360684..363098 (-) 2415 WP_193035662.1 type II secretion system secretin GspD -
  HF909_RS01700 (HF909_01715) - 363132..363983 (-) 852 WP_193035664.1 type II secretion system protein N -
  HF909_RS01705 (HF909_01720) gspM 363987..364565 (-) 579 WP_193027253.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55809.82 Da        Isoelectric Point: 5.3667

>NTDB_id=382926 HF909_RS01690 WP_193035660.1 359093..360649(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2474]
MATQASSTDARTLETPSPSAVRLVPYVFARDAKLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLLSLDAATLSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLARFDHLINQPHGIVLVTGPTGSGKTTTLYAALSRLDAGSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGVAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAAES
EIKALAIGQGMQTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=382926 HF909_RS01690 WP_193035660.1 359093..360649(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2474]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGACGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTACGT
GTTCGCGCGCGACGCCAAGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGTTGAGCCTGGATGCCGCCACGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTGGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGATCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCGCGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCTTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAAGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGGCCGGTGGACGTGC
GCGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGTCTGCTCGACAAGGAGGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGCGCGCTTCGACCACCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCGGCAGCACCAACATCATGA
CGGTCGAGGACCCGATCGAGTACGACCTGGACGGCATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGATCCGGACGTGGTGATGATCGGCGAAATCCGCGACCTGGAGACCGCGCAGAT
CGCAGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAACTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCTGA
CGGCGTTGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGTTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGGCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCAGACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGATTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.434

86.68

0.394
  pilB Vibrio campbellii strain DS40M4

45.212

86.68

0.392
  pilF Thermus thermophilus HB27

46.539

80.888

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365

Multiple sequence alignment