Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GALF_RS01445 Genome accession   NC_014394
Coordinates   329954..331351 (+) Length   465 a.a.
NCBI ID   WP_013292268.1    Uniprot ID   -
Organism   Gallionella capsiferriformans ES-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324954..336351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GALF_RS01420 (Galf_0275) - 326540..327526 (+) 987 WP_013292263.1 SIS domain-containing protein -
  GALF_RS01425 (Galf_0276) - 327575..328093 (+) 519 WP_013292264.1 HAD family hydrolase -
  GALF_RS01430 (Galf_0277) lptC 328090..328665 (+) 576 WP_013292265.1 LPS export ABC transporter periplasmic protein LptC -
  GALF_RS01435 (Galf_0278) lptA 328655..329191 (+) 537 WP_013292266.1 lipopolysaccharide transport periplasmic protein LptA -
  GALF_RS01440 (Galf_0279) lptB 329188..329910 (+) 723 WP_013292267.1 LPS export ABC transporter ATP-binding protein -
  GALF_RS01445 (Galf_0280) rpoN/rpoN1 329954..331351 (+) 1398 WP_013292268.1 RNA polymerase factor sigma-54 Machinery gene
  GALF_RS01450 (Galf_0281) hpf 331367..331687 (+) 321 WP_013292269.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GALF_RS01455 (Galf_0282) - 331737..332198 (+) 462 WP_013292270.1 PTS sugar transporter subunit IIA -
  GALF_RS01460 (Galf_0283) hprK 332195..333133 (+) 939 WP_013292271.1 HPr(Ser) kinase/phosphatase -
  GALF_RS01465 (Galf_0284) rapZ 333136..333972 (+) 837 WP_013292272.1 RNase adapter RapZ -
  GALF_RS01470 (Galf_0285) - 333977..334357 (+) 381 WP_013292273.1 NusG domain II-containing protein -
  GALF_RS01475 (Galf_0286) - 334338..334955 (+) 618 WP_396230358.1 Gx transporter family protein -
  GALF_RS01480 (Galf_0287) - 334971..335603 (+) 633 WP_013292275.1 flavin prenyltransferase UbiX -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52014.36 Da        Isoelectric Point: 4.7787

>NTDB_id=38095 GALF_RS01445 WP_013292268.1 329954..331351(+) (rpoN/rpoN1) [Gallionella capsiferriformans ES-2]
MKASLQLRMSQQLTLTPQLQQSIRLLQLSTLDMQQEIDRMLDENPMLELGDESVYHALSGEPVREDHAQVDNAESERGEA
EPFEWNAETRGADDESDAYPEQAALQASMREYLHSQLSVSQLDSRDRLVIGMLIDALDENGYMLQDLAELAGLLPAELEL
TLDDLETALVQLQYLDRPGLGARTLSECLALQLKALPEQTPGRDLAMSLVNGNLDLLAAHDFSKIKKLLRCSDASLRVAQ
ELIVSLNPKPGCEFDRSVADYVVPDVIVEQHKGKWRARLNADAMPRLRVNQMYANILQQRDDKSGAPLVSQLQEASWLIK
SLRQRFDTILRVAQAIVDRQGAFLEHGDIAMRPLVLREIADVLEMHESTVSRGTTQKFMRTPRGIYEFKYFFGSGLVTES
GGVCSSAAIRELIKQLVGGEDTSKPLTDGRMSEILAQQGIVVARRTIAKYREALHILPVNLRKSL

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=38095 GALF_RS01445 WP_013292268.1 329954..331351(+) (rpoN/rpoN1) [Gallionella capsiferriformans ES-2]
ATGAAAGCCTCTCTACAACTTCGCATGTCTCAGCAACTGACGCTCACGCCTCAGTTGCAACAATCCATTCGCCTACTTCA
GCTCTCTACGCTGGATATGCAACAGGAAATTGATCGGATGCTCGATGAAAATCCGATGCTGGAATTGGGTGATGAGTCCG
TATACCACGCATTATCAGGCGAGCCTGTTCGTGAAGATCATGCACAGGTGGATAATGCCGAATCGGAACGCGGCGAGGCT
GAGCCGTTTGAGTGGAATGCAGAGACGCGTGGCGCTGATGATGAAAGCGACGCCTATCCCGAACAGGCGGCGCTGCAGGC
CAGCATGCGGGAGTATTTGCACAGCCAGTTGTCAGTGAGTCAACTGGATAGCCGGGATAGGCTGGTGATCGGGATGCTGA
TCGATGCGCTCGATGAAAATGGTTACATGTTGCAGGATTTGGCGGAATTGGCCGGGTTGTTGCCGGCGGAACTGGAGTTG
ACGCTAGATGATCTTGAAACGGCGCTCGTTCAGTTGCAGTATCTTGATCGTCCGGGGCTTGGGGCGCGGACGTTAAGTGA
ATGTCTGGCGTTGCAGTTGAAGGCGTTGCCAGAGCAGACGCCGGGGCGTGATTTGGCGATGAGCCTGGTGAACGGGAATC
TTGATCTGCTGGCCGCACACGATTTTTCCAAAATTAAAAAACTGCTGCGTTGCTCCGATGCCAGTTTGCGTGTGGCACAG
GAATTGATTGTCAGCCTGAACCCTAAGCCCGGTTGCGAGTTTGACCGCAGCGTTGCCGATTACGTTGTGCCGGATGTGAT
TGTCGAGCAGCACAAGGGTAAATGGCGCGCTAGGCTGAATGCCGATGCGATGCCTCGCCTGCGCGTCAATCAGATGTATG
CGAATATTTTGCAGCAGCGCGATGATAAATCGGGTGCGCCGCTGGTGTCGCAATTGCAGGAAGCGAGCTGGCTGATTAAG
AGTCTGCGGCAGCGTTTTGATACTATATTGCGGGTCGCGCAGGCGATTGTCGATCGGCAGGGGGCTTTTCTTGAACACGG
TGACATCGCCATGCGCCCGCTGGTATTGCGCGAAATAGCCGATGTGCTTGAGATGCACGAGTCAACTGTTTCACGCGGTA
CGACGCAAAAGTTTATGCGTACGCCGCGTGGCATTTATGAATTTAAATATTTCTTTGGCAGCGGTCTGGTCACGGAAAGT
GGCGGCGTCTGTTCTTCTGCCGCAATACGCGAACTGATTAAACAACTGGTCGGCGGTGAAGATACAAGCAAGCCACTAAC
GGACGGCCGAATGTCAGAAATCTTGGCGCAGCAGGGCATAGTCGTCGCCCGACGCACCATAGCAAAGTATCGGGAAGCGT
TGCATATACTCCCGGTGAATCTCCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(102-293)

Sigma-54, DNA binding domain (Sigma54_DBD)

(310-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.098

100

0.551

Multiple sequence alignment