Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BMF77_RS20810 Genome accession   NZ_CP043056
Coordinates   4372295..4373812 (+) Length   505 a.a.
NCBI ID   WP_148765485.1    Uniprot ID   -
Organism   Dolichospermum sp. UHCC 0315A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4367295..4378812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF77_RS20785 (BMF77_04056) crtO 4368067..4369755 (+) 1689 WP_015079767.1 beta-carotene ketolase CrtO -
  BMF77_RS20790 (BMF77_04057) - 4369772..4370290 (-) 519 WP_148765479.1 DUF456 domain-containing protein -
  BMF77_RS20795 (BMF77_04058) - 4370420..4371067 (-) 648 WP_148765481.1 cofactor assembly of complex C subunit B -
  BMF77_RS26915 - 4370971..4371414 (+) 444 WP_168204366.1 hypothetical protein -
  BMF77_RS20805 (BMF77_04059) rpaB 4371394..4372122 (-) 729 WP_148765483.1 response regulator transcription factor RpaB -
  BMF77_RS20810 (BMF77_04060) radA 4372295..4373812 (+) 1518 WP_148765485.1 DNA repair protein RadA Machinery gene
  BMF77_RS20815 (BMF77_04061) - 4373818..4375101 (-) 1284 WP_148765487.1 RNA-guided endonuclease TnpB family protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 54412.22 Da        Isoelectric Point: 5.0043

>NTDB_id=380416 BMF77_RS20810 WP_148765485.1 4372295..4373812(+) (radA) [Dolichospermum sp. UHCC 0315A]
MAKPKTIYICSSCAAEFSQWFGKCSNCDTYDSLVEQNISSFIGDIPSRSGVGNWQAGGHSKSHNKPAKARSSLTFDQISD
RQIARWESGYGELDRVLGGGIVPGSMVLIGGDPGIGKSTLLLQVSNQLAQKYRILYVTGEESGQQVKLRASRLGMSKPPT
LVTEDPENKDPEKPIDPDSIGANLYVLPETDLEEILREIDSLKPNVAVIDSIQTVFFPALTSAPGSVAQVRECTAALMKV
AKHEDITMLIVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFASHRLLRTVKNRFGATHEIGIFEMVSQGLKEVDNPSELF
LGNRDDPAPGTAIVVACEGTRPIVVELQALVSPTSYPSPRRAGTGIDYNRLVQILAVLEKRVGIPMSKLDSYVASAGGLN
VEEPAVDLGIAVAIVASFRDRIVDPGTVLIGEVGLGGQVRSVSQMELRLKEAAKLGFKRAIVPKGTKFPEIGIEILPVGK
VIDAIIAAVPHQELTEEDLDPDENE

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=380416 BMF77_RS20810 WP_148765485.1 4372295..4373812(+) (radA) [Dolichospermum sp. UHCC 0315A]
ATGGCAAAGCCAAAAACCATTTATATCTGTAGTAGTTGTGCAGCGGAATTCTCTCAATGGTTCGGAAAATGTTCAAATTG
TGACACTTACGATTCTTTAGTTGAGCAAAATATTAGTTCTTTTATCGGGGATATACCTAGCCGGTCTGGAGTGGGAAATT
GGCAAGCTGGAGGTCATAGTAAGTCTCATAATAAACCAGCTAAGGCCAGATCTTCTCTGACTTTTGACCAAATTAGCGAT
CGCCAAATTGCTCGTTGGGAGTCCGGTTATGGGGAATTAGATCGAGTTTTAGGTGGTGGAATTGTTCCCGGTTCGATGGT
GCTAATTGGTGGCGACCCTGGTATCGGTAAATCTACTTTACTACTGCAAGTTTCTAACCAATTGGCGCAAAAATACCGAA
TTCTCTATGTGACTGGAGAAGAATCTGGACAACAGGTAAAATTGCGTGCTTCTCGTTTGGGAATGTCAAAACCACCTACG
CTAGTCACGGAAGATCCTGAAAACAAAGATCCGGAAAAACCGATAGATCCTGACAGTATAGGTGCAAATTTGTATGTTTT
GCCAGAAACGGATTTAGAAGAAATTCTGAGGGAAATAGATTCTCTAAAACCAAATGTAGCAGTAATAGATAGTATCCAAA
CAGTGTTTTTTCCTGCTTTGACATCAGCACCGGGTTCAGTTGCTCAAGTCAGGGAATGTACCGCAGCTTTGATGAAGGTG
GCAAAACATGAAGATATTACGATGTTAATTGTGGGTCATGTTACCAAAGAAGGAGCGATCGCCGGTCCCAAAGTATTAGA
ACATTTAGTAGATACCGTATTATATTTTGAAGGCGATCGCTTTGCCTCCCACAGATTATTAAGAACTGTAAAAAACCGTT
TCGGAGCAACCCACGAAATTGGTATATTTGAAATGGTATCCCAAGGATTAAAGGAAGTTGATAATCCTTCCGAACTGTTT
TTAGGCAATCGTGATGATCCGGCTCCGGGTACGGCTATTGTGGTGGCTTGTGAAGGAACAAGACCAATAGTTGTGGAATT
ACAGGCTTTAGTCAGTCCTACCAGTTACCCATCTCCTCGTCGGGCGGGTACAGGCATAGATTATAATCGCTTAGTGCAGA
TTCTCGCCGTCTTAGAAAAGCGTGTAGGGATACCTATGTCCAAATTAGATTCCTACGTTGCTTCTGCGGGGGGATTAAAT
GTAGAAGAACCAGCGGTAGACTTAGGAATTGCAGTAGCGATAGTTGCCAGTTTCCGCGATCGAATCGTTGATCCTGGTAC
AGTCTTAATTGGGGAAGTAGGGTTAGGGGGACAAGTGCGGTCGGTTTCCCAAATGGAACTACGCTTAAAAGAAGCCGCGA
AACTAGGATTTAAAAGAGCCATAGTTCCTAAAGGGACAAAATTTCCTGAGATTGGGATTGAGATACTACCAGTAGGTAAA
GTCATAGATGCAATTATTGCGGCTGTTCCTCATCAAGAATTGACAGAAGAAGATTTAGACCCGGATGAGAATGAATAA

Domains

Predicted by InterproScan.

KaiC (ATPase)

(89-293)

Subunit ChlI of Mg-chelatase (ChlI)

(399-464)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.102

97.228

0.487
  radA Streptococcus mitis NCTC 12261

46.203

93.861

0.434
  radA Streptococcus pneumoniae Rx1

46.203

93.861

0.434
  radA Streptococcus pneumoniae D39

46.203

93.861

0.434
  radA Streptococcus pneumoniae R6

46.203

93.861

0.434
  radA Streptococcus pneumoniae TIGR4

46.203

93.861

0.434
  radA Streptococcus mitis SK321

46.203

93.861

0.434

Multiple sequence alignment