Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   FXN57_RS00365 Genome accession   NZ_CP043005
Coordinates   73224..73769 (+) Length   181 a.a.
NCBI ID   WP_148363051.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain PN_696     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68224..78769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN57_RS00350 (FXN57_00350) - 69174..70538 (+) 1365 WP_148323211.1 L-serine ammonia-lyase -
  FXN57_RS00355 (FXN57_00355) ansB 70718..71767 (-) 1050 WP_148363050.1 L-asparaginase 2 -
  FXN57_RS00360 (FXN57_00360) - 72105..73199 (+) 1095 WP_148323215.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FXN57_RS00365 (FXN57_00365) pilF/pilF2 73224..73769 (+) 546 WP_148363051.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FXN57_RS00370 (FXN57_00370) - 73918..74973 (+) 1056 WP_148363052.1 RodZ family helix-turn-helix domain-containing protein -
  FXN57_RS00375 (FXN57_00375) ispG 74985..76088 (+) 1104 WP_005547472.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FXN57_RS00380 (FXN57_00380) hisS 76110..77387 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  FXN57_RS00385 (FXN57_00385) - 77398..78012 (+) 615 WP_005539337.1 YfgM family protein -
  FXN57_RS00390 (FXN57_00390) - 78065..78517 (+) 453 WP_148323225.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20386.12 Da        Isoelectric Point: 8.0744

>NTDB_id=380152 FXN57_RS00365 WP_148363051.1 73224..73769(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain PN_696]
MIKFQQQNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKACSYAPDYYLVSAAFAYF
YQQQGDIEQVRAAYLKAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPERAAKLPQG

Nucleotide


Download         Length: 546 bp        

>NTDB_id=380152 FXN57_RS00365 WP_148363051.1 73224..73769(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain PN_696]
ATGATCAAATTTCAACAACAAAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAACAAAAAGCCGCCAAAGCACGCGTAGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTGTTCTTACGCTCCAGATTATTATTTAGTATCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGTCCGTGCCGCCTATTTAAAAGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTTGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGCTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCCCCGGAACGGGCGGCAAAATTACCACAAGGATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

56.364

91.16

0.514
  pilF Glaesserella parasuis strain SC1401

46.471

93.923

0.436

Multiple sequence alignment