Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   FXN58_RS00375 Genome accession   NZ_CP043003
Coordinates   75877..76422 (+) Length   181 a.a.
NCBI ID   WP_148323217.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain HK_907     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70877..81422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN58_RS00360 (FXN58_00360) - 71827..73191 (+) 1365 WP_148323211.1 L-serine ammonia-lyase -
  FXN58_RS00365 (FXN58_00365) ansB 73371..74420 (-) 1050 WP_148323213.1 L-asparaginase 2 -
  FXN58_RS00370 (FXN58_00370) - 74758..75852 (+) 1095 WP_148323215.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FXN58_RS00375 (FXN58_00375) pilF/pilF2 75877..76422 (+) 546 WP_148323217.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FXN58_RS00380 (FXN58_00380) - 76571..77626 (+) 1056 WP_148323219.1 RodZ family helix-turn-helix domain-containing protein -
  FXN58_RS00385 (FXN58_00385) ispG 77638..78741 (+) 1104 WP_187386207.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FXN58_RS00390 (FXN58_00390) hisS 78763..80040 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  FXN58_RS00395 (FXN58_00395) - 80051..80665 (+) 615 WP_148323223.1 YfgM family protein -
  FXN58_RS00400 (FXN58_00400) - 80718..81170 (+) 453 WP_148323225.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20434.20 Da        Isoelectric Point: 8.4086

>NTDB_id=380108 FXN58_RS00375 WP_148323217.1 75877..76422(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain HK_907]
MIKFQQQNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKACSYAPDYYLVSAAFAYF
YQQQGDIEQVRAAYLKAIKLYDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPERAAKLPQG

Nucleotide


Download         Length: 546 bp        

>NTDB_id=380108 FXN58_RS00375 WP_148323217.1 75877..76422(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain HK_907]
ATGATCAAATTTCAACAACAAAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAACAAAAAGCCGCCAAAGCACGCGTAGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTGTTCTTACGCTCCAGATTATTATTTAGTATCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGTCCGTGCCGCCTATTTAAAAGCGATAAAATTATATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTTGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGCTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCCCCGGAACGGGCGGCAAAATTACCACAAGGATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

56.364

91.16

0.514
  pilF Glaesserella parasuis strain SC1401

46.471

93.923

0.436

Multiple sequence alignment