Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   FWK61_RS06685 Genome accession   NZ_CP042992
Coordinates   1498484..1499023 (-) Length   179 a.a.
NCBI ID   WP_075321386.1    Uniprot ID   -
Organism   Histophilus somni strain UOC-KLM-ATR-013     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1493484..1504023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK61_RS09875 - 1494915..1495061 (-) 147 WP_157665520.1 hypothetical protein -
  FWK61_RS06670 (FWK61_06670) - 1495061..1495276 (-) 216 WP_075294140.1 transposase -
  FWK61_RS06675 (FWK61_06675) - 1495385..1495642 (+) 258 WP_075294154.1 helix-turn-helix domain-containing protein -
  FWK61_RS06680 (FWK61_06680) nrdA 1496131..1498401 (+) 2271 WP_011609351.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  FWK61_RS06685 (FWK61_06685) pilF/pilF2 1498484..1499023 (-) 540 WP_075321386.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FWK61_RS06690 (FWK61_06690) - 1499054..1500172 (-) 1119 WP_132995224.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FWK61_RS06695 (FWK61_06695) dnaX 1500425..1502428 (-) 2004 WP_132995220.1 DNA polymerase III subunit gamma/tau -
  FWK61_RS06700 (FWK61_06700) apt 1502459..1502998 (-) 540 WP_012341218.1 adenine phosphoribosyltransferase -
  FWK61_RS06705 (FWK61_06705) lpxM 1503069..1504022 (-) 954 WP_132995218.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20682.64 Da        Isoelectric Point: 9.3139

>NTDB_id=379984 FWK61_RS06685 WP_075321386.1 1498484..1499023(-) (pilF/pilF2) [Histophilus somni strain UOC-KLM-ATR-013]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALITPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=379984 FWK61_RS06685 WP_075321386.1 1498484..1499023(-) (pilF/pilF2) [Histophilus somni strain UOC-KLM-ATR-013]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTATTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATCATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

43.503

98.883

0.43

Multiple sequence alignment