Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FWK50_RS07810 Genome accession   NZ_CP042989
Coordinates   1742760..1744175 (-) Length   471 a.a.
NCBI ID   WP_232451458.1    Uniprot ID   -
Organism   Histophilus somni strain UOC-KLM-ATR-010     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737760..1749175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK50_RS07795 (FWK50_07795) ffh 1739074..1740450 (-) 1377 WP_075293626.1 signal recognition particle protein -
  FWK50_RS07800 (FWK50_07800) - 1740600..1741397 (+) 798 WP_075293627.1 inner membrane protein YpjD -
  FWK50_RS07805 (FWK50_07805) - 1741467..1742726 (+) 1260 WP_012341088.1 HlyC/CorC family transporter -
  FWK50_RS07810 (FWK50_07810) pilB 1742760..1744175 (-) 1416 WP_232451458.1 GspE/PulE family protein Machinery gene
  FWK50_RS07815 (FWK50_07815) tig 1744534..1745832 (+) 1299 WP_075293628.1 trigger factor -
  FWK50_RS07820 (FWK50_07820) clpP 1745918..1746499 (+) 582 WP_075293629.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  FWK50_RS07825 (FWK50_07825) clpX 1746515..1747759 (+) 1245 WP_075293630.1 ATP-dependent protease ATP-binding subunit ClpX -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 53852.80 Da        Isoelectric Point: 5.9894

>NTDB_id=379914 FWK50_RS07810 WP_232451458.1 1742760..1744175(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-010]
MIIPQSEDMSPIVMSIDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPV
LLNNQLLKQLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQNVSDIHIEPQMNCLQIRFR
IDGILQCQSPLPFSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLS
FAELGMTENQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDF
SRLLRTFLRQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRK
KCPHTENENCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSSE

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=379914 FWK50_RS07810 WP_232451458.1 1742760..1744175(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-010]
ATGATAATTCCTCAATCTGAAGATATGTCTCCGATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACCGCATTT
ATGGCAAAGAAATCAGCAACAATCCCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTAT
GGTTAGGCGTTGATAGTCTAAATAATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTT
TTACTTAATAACCAATTACTTAAACAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACC
TATAAATCAATATGTAGCCGAGCATCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACA
TTTTTGAAAATGCGTTAAAGCAAAATGTATCAGATATTCATATTGAACCACAAATGAATTGCTTGCAAATACGTTTCAGA
ATAGACGGTATTTTACAATGCCAATCGCCTCTTCCGTTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGC
CAAATTGGATATAAGCGAAACACGGTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATT
TTCGTTTATCAACCCTTCCGACGAATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGT
TTTGCGGAACTCGGTATGACAGAAAACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGT
GACAGGCCCAACAGGGAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTGAATGATAAGCACAAACATATTA
TGACAGCGGAAGATCCGATAGAAATTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTT
AGCCGACTGTTAAGAACTTTTCTTCGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAAT
AATGGCATTAAGAGCAGCACAAACCGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTCTCAC
GCTTGCTTCAATTGGGGGTAAAGCAACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAA
AAATGTCCACATACAGAAAATGAAAATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACA
ACCGCATCTAATAGATAATCATATTTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGT
TAGAAATAACAGATTTAGCAGAAGTGGATAGAGTTATTGGACAAAGTAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-98)

Type II/IV secretion system protein (T2SSE)

(134-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.547

97.877

0.671
  pilB Haemophilus influenzae Rd KW20

67.826

97.665

0.662
  pilB Glaesserella parasuis strain SC1401

57.556

95.541

0.55
  pilB Vibrio campbellii strain DS40M4

40.041

100

0.41
  pilB Vibrio cholerae strain A1552

39.212

100

0.401
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.401
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.389
  pilB Acinetobacter baumannii D1279779

37.267

100

0.382
  pilB Acinetobacter baylyi ADP1

35.928

100

0.382
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.361

Multiple sequence alignment