Detailed information NTG0378371

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FR848_RS24670
• Genome accession: NZ_CP042545
• Coordinates: 5182792..5183589 (+)
• Length: 265 a.a.
• NCBI ID: WP_032694399.1
• Uniprot ID: A0A2J5PD60
• Organism: Klebsiella michiganensis strain C52
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5177792..5188589

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FR848_RS24635 (FR848_24645)	gspH	5177933..5178445 (+)	513	WP_038424080.1	type II secretion system minor pseudopilin GspH	-
FR848_RS24640 (FR848_24650)	gspI	5178442..5178807 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
FR848_RS24645 (FR848_24655)	gspJ	5178804..5179400 (+)	597	WP_014837428.1	type II secretion system minor pseudopilin GspJ	-
FR848_RS24650 (FR848_24660)	gspK	5179397..5180377 (+)	981	WP_032720565.1	type II secretion system minor pseudopilin GspK	-
FR848_RS24655 (FR848_24665)	gspL	5180370..5181563 (+)	1194	WP_032720564.1	type II secretion system protein GspL	-
FR848_RS24660 (FR848_24670)	gspM	5181556..5182041 (+)	486	WP_038424078.1	type II secretion system protein GspM	-
FR848_RS24665 (FR848_24675)	-	5182031..5182789 (+)	759	WP_038424077.1	type II secretion system protein N	-
FR848_RS24670 (FR848_24680)	pilD	5182792..5183589 (+)	798	WP_032694399.1	prepilin peptidase	Machinery gene
FR848_RS24675 (FR848_24685)	hrpB	5183687..5186116 (-)	2430	WP_038424076.1	ATP-dependent helicase HrpB	-
FR848_RS24680 (FR848_24690)	thpR	5186189..5186725 (+)	537	WP_014227968.1	RNA 2',3'-cyclic phosphodiesterase	-
FR848_RS24685 (FR848_24695)	sfsA	5186725..5187441 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
FR848_RS24690 (FR848_24700)	dksA	5187606..5188061 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29150.77 Da
• Isoelectric Point: 8.3410

>NTDB_id=378371 FR848_RS24670 WP_032694399.1 5182792..5183589(+) (pilD) [Klebsiella michiganensis strain C52]
MENIALLPEFAAQFPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=378371 FR848_RS24670 WP_032694399.1 5182792..5183589(+) (pilD) [Klebsiella michiganensis strain C52]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTAAATCTCTGCC
TCCCCGCCTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A2J5PD60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.808	98.491	0.491
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434