Detailed information NTG0378108

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FR751_RS06150
• Genome accession: NZ_CP042481
• Coordinates: 1302350..1303150 (-)
• Length: 266 a.a.
• NCBI ID: WP_040089576.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain C51
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1297350..1308150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FR751_RS06130 (FR751_06130)	dksA	1297930..1298385 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FR751_RS06135 (FR751_06135)	sfsA	1298548..1299264 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FR751_RS06140 (FR751_06140)	thpR	1299264..1299800 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
FR751_RS06145 (FR751_06145)	hrpB	1299873..1302302 (+)	2430	WP_040089577.1	ATP-dependent helicase HrpB	-
FR751_RS06150 (FR751_06150)	pilD	1302350..1303150 (-)	801	WP_040089576.1	prepilin peptidase	Machinery gene
FR751_RS06155 (FR751_06155)	-	1303147..1303893 (-)	747	WP_040089575.1	type II secretion system protein N	-
FR751_RS06160 (FR751_06160)	-	1303883..1304368 (-)	486	WP_023284693.1	type II secretion system protein M	-
FR751_RS06165 (FR751_06165)	gspL	1304361..1305554 (-)	1194	WP_025985182.1	type II secretion system protein GspL	-
FR751_RS06170 (FR751_06170)	gspK	1305541..1306527 (-)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
FR751_RS06175 (FR751_06175)	gspJ	1306524..1307120 (-)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
FR751_RS06180 (FR751_06180)	gspI	1307117..1307482 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FR751_RS06185 (FR751_06185)	gspH	1307479..1307994 (-)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29354.99 Da
• Isoelectric Point: 8.8894

>NTDB_id=378108 FR751_RS06150 WP_040089576.1 1302350..1303150(-) (pilD) [Klebsiella pneumoniae strain C51]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=378108 FR751_RS06150 WP_040089576.1 1302350..1303150(-) (pilD) [Klebsiella pneumoniae strain C51]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425