Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FR741_RS01750 Genome accession   NZ_CP042451
Coordinates   362289..363305 (+) Length   338 a.a.
NCBI ID   WP_046874633.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FA3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 357289..368305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR741_RS01735 (FR741_01825) - 357722..358450 (-) 729 WP_005395721.1 redoxin family protein -
  FR741_RS01740 (FR741_01835) oxyR 358621..359532 (+) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  FR741_RS01745 (FR741_01840) - 359584..362145 (-) 2562 WP_104974736.1 penicillin-binding protein 1A -
  FR741_RS01750 (FR741_01845) pilM 362289..363305 (+) 1017 WP_046874633.1 type IV pilus biogenesis protein PilM Machinery gene
  FR741_RS01755 (FR741_01850) pilN 363289..363864 (+) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  FR741_RS01760 (FR741_01855) pilO 363857..364450 (+) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  FR741_RS01765 (FR741_01860) pilP 364440..364955 (+) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  FR741_RS01770 (FR741_01865) pilQ 364981..366729 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  FR741_RS01775 (FR741_01870) aroK 366917..367435 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37690.10 Da        Isoelectric Point: 7.2093

>NTDB_id=377976 FR741_RS01750 WP_046874633.1 362289..363305(+) (pilM) [Vibrio diabolicus strain FA3]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQNDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=377976 FR741_RS01750 WP_046874633.1 362289..363305(+) (pilM) [Vibrio diabolicus strain FA3]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAATTGCAAATAGAACAAAACCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACACGCGGAGCAAACAGCCCTGTTCAAAATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

51.045

99.112

0.506

Multiple sequence alignment