Detailed information NTG0037772

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NWAT_RS06085
• Genome accession: NC_014315
• Coordinates: 1326320..1327492 (-)
• Length: 390 a.a.
• NCBI ID: WP_013220260.1
• Uniprot ID: D8K5I7
• Organism: Nitrosococcus watsonii C-113
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1321320..1332492

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NWAT_RS06065 (Nwat_1229)	-	1322015..1323337 (+)	1323	WP_013220256.1	PhoPQ-activated pathogenicity-related family protein	-
NWAT_RS06070 (Nwat_1230)	-	1323367..1324569 (+)	1203	WP_013220257.1	ChaN family lipoprotein	-
NWAT_RS06075 (Nwat_1231)	-	1324751..1325194 (+)	444	WP_041350534.1	YcgN family cysteine cluster protein	-
NWAT_RS06080 (Nwat_1232)	-	1325449..1325910 (-)	462	WP_013220259.1	hypothetical protein	-
NWAT_RS06085 (Nwat_1233)	pilU	1326320..1327492 (-)	1173	WP_013220260.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NWAT_RS06090 (Nwat_1234)	-	1327620..1328543 (+)	924	WP_013220261.1	AEC family transporter	-
NWAT_RS06095 (Nwat_1235)	-	1328536..1329282 (-)	747	WP_013220262.1	molybdopterin-binding protein	-
NWAT_RS06100 (Nwat_1236)	-	1329386..1331308 (+)	1923	WP_013220263.1	ATP-dependent DNA helicase	-
NWAT_RS06105 (Nwat_1237)	-	1331221..1332255 (-)	1035	WP_013220264.1	bile acid:sodium symporter family protein	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43372.79 Da
• Isoelectric Point: 6.9783

>NTDB_id=37772 NWAT_RS06085 WP_013220260.1 1326320..1327492(-) (pilU) [Nitrosococcus watsonii C-113]
MEFKDYLKILVAHDGSDIYLSAGAPPCAKFHGLLKPLEKTRLSSSDIKKIAYDIMNEEQASEFEQKPEMNLALSESGIGR
FRVNIFKQRSAISMVIRNIKTTIPNVDNLGLPPILKNVVMDKHGLILFVGSTGSGKSTSLAALIDHRNNSSAGHIITIED
PIEYIHHHKRSIVNQREIGVDTESYEDALKNTLRQAPDVILIGEIRDRSTMEHAISFAETGHLCLSTLHANNANQALDRI
INFFPEDRRPQLLLDLSLNLRAFISQRLIPTIDGKRTAAIEILLGTPLVRDLIHRGEIHAIKEVMEKSENLGMQTFDSAL
YKLYTAGLISVEEALRNADSLNNLRLKINLSKKGMEATTYSPAAEKNSMDKKPKKDVLGGLSFSLVGEED

Nucleotide

• Length: 1173 bp

>NTDB_id=37772 NWAT_RS06085 WP_013220260.1 1326320..1327492(-) (pilU) [Nitrosococcus watsonii C-113]
ATGGAATTTAAGGATTACCTAAAAATACTTGTTGCTCATGATGGCTCGGATATTTACCTGAGTGCCGGAGCCCCCCCCTG
CGCCAAATTCCACGGTTTGCTGAAACCTCTCGAAAAAACGAGATTATCTTCCAGCGATATAAAGAAGATCGCCTACGATA
TTATGAACGAAGAACAAGCAAGCGAGTTTGAACAAAAGCCGGAAATGAACTTGGCCCTTTCCGAATCTGGCATTGGACGC
TTTCGAGTCAATATTTTCAAACAGCGCAGCGCAATTTCCATGGTTATCCGGAATATCAAAACTACCATCCCTAACGTAGA
TAACCTCGGCCTGCCTCCTATATTAAAAAACGTTGTTATGGATAAGCATGGCCTCATTTTATTTGTAGGGTCAACCGGTT
CCGGTAAGTCGACCTCCCTTGCCGCCCTCATTGATCATCGCAATAATAGCAGCGCGGGTCATATCATCACGATTGAAGAC
CCCATCGAGTATATACACCACCATAAAAGATCCATCGTCAATCAGCGCGAAATAGGAGTCGACACGGAATCTTATGAAGA
TGCACTTAAAAATACCCTACGCCAGGCACCGGATGTTATTCTTATTGGAGAAATCCGTGACCGAAGTACTATGGAACATG
CCATCTCCTTTGCGGAAACGGGTCACTTATGCCTCTCTACCCTTCATGCTAATAATGCTAACCAGGCGCTTGACCGAATT
ATCAACTTCTTCCCGGAAGACCGGCGCCCACAGTTACTATTAGATCTCTCTTTAAATCTACGCGCTTTTATTTCCCAACG
TCTGATTCCTACCATTGATGGCAAGCGCACCGCGGCTATTGAAATTCTATTGGGAACGCCTCTGGTGCGAGACCTTATCC
ACCGGGGGGAAATCCACGCTATTAAGGAGGTTATGGAAAAATCAGAAAATCTTGGTATGCAAACCTTCGACAGCGCCCTT
TATAAACTGTATACAGCCGGCCTTATTAGCGTTGAAGAGGCACTGCGTAACGCCGATTCGCTTAATAACCTCCGCCTCAA
AATTAACTTGTCAAAAAAAGGAATGGAAGCGACCACCTATTCTCCCGCAGCGGAAAAAAACTCCATGGATAAGAAACCAA
AAAAGGATGTATTAGGCGGGCTATCCTTTAGCTTGGTGGGAGAAGAAGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (47-269)

3D structure

• AlphaFold DB: D8K5I7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.556	96.923	0.538
pilU	Acinetobacter baylyi ADP1	55.618	91.282	0.508
pilU	Vibrio cholerae strain A1552	51.268	91.026	0.467
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.466	93.59	0.397