Detailed information NTG0377043

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FRX36_RS10255
• Genome accession: NZ_CP042321
• Coordinates: 2100604..2101404 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 1-16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2095604..2106404

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FRX36_RS10235	dksA	2096184..2096639 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FRX36_RS10240	sfsA	2096802..2097518 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FRX36_RS10245	thpR	2097518..2098054 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
FRX36_RS10250	hrpB	2098127..2100556 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
FRX36_RS10255	pilD	2100604..2101404 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
FRX36_RS10260	-	2101401..2102147 (-)	747	WP_004145897.1	type II secretion system protein N	-
FRX36_RS10265	-	2102137..2102622 (-)	486	WP_004151943.1	type II secretion system protein M	-
FRX36_RS10270	gspL	2102615..2103808 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
FRX36_RS10275	gspK	2103795..2104781 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
FRX36_RS10280	gspJ	2104778..2105374 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
FRX36_RS10285	gspI	2105371..2105736 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FRX36_RS10290	gspH	2105733..2106248 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=377043 FRX36_RS10255 WP_002888947.1 2100604..2101404(-) (pilD) [Klebsiella pneumoniae strain 1-16]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=377043 FRX36_RS10255 WP_002888947.1 2100604..2101404(-) (pilD) [Klebsiella pneumoniae strain 1-16]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425