Detailed information NTG0377019

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FLM25_RS10265
• Genome accession: NZ_CP042320
• Coordinates: 2105465..2106265 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 5-16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2100465..2111265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLM25_RS10245	dksA	2101045..2101500 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FLM25_RS10250	sfsA	2101663..2102379 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FLM25_RS10255	thpR	2102379..2102915 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
FLM25_RS10260	hrpB	2102988..2105417 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
FLM25_RS10265	pilD	2105465..2106265 (-)	801	WP_002888947.1	A24 family peptidase	Machinery gene
FLM25_RS10270	-	2106262..2107008 (-)	747	WP_004145897.1	type II secretion system protein N	-
FLM25_RS10275	-	2106998..2107483 (-)	486	WP_004151943.1	type II secretion system protein M	-
FLM25_RS10280	gspL	2107476..2108669 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
FLM25_RS10285	gspK	2108656..2109642 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
FLM25_RS10290	gspJ	2109639..2110235 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
FLM25_RS10295	gspI	2110232..2110597 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FLM25_RS10300	gspH	2110594..2111109 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=377019 FLM25_RS10265 WP_002888947.1 2105465..2106265(-) (pilD) [Klebsiella pneumoniae strain 5-16]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=377019 FLM25_RS10265 WP_002888947.1 2105465..2106265(-) (pilD) [Klebsiella pneumoniae strain 5-16]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425