Detailed information NTG0376924

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FLM33_RS09745
• Genome accession: NZ_CP042315
• Coordinates: 2003585..2004385 (-)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 89-17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1998585..2009385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLM33_RS09725	dksA	1999165..1999620 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FLM33_RS09730	sfsA	1999783..2000499 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
FLM33_RS09735	thpR	2000499..2001035 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
FLM33_RS09740	hrpB	2001108..2003537 (+)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
FLM33_RS09745	pilD	2003585..2004385 (-)	801	WP_004213441.1	A24 family peptidase	Machinery gene
FLM33_RS09750	-	2004382..2005128 (-)	747	WP_032434210.1	type II secretion system protein N	-
FLM33_RS09755	-	2005118..2005603 (-)	486	WP_004145896.1	type II secretion system protein M	-
FLM33_RS09760	gspL	2005596..2006789 (-)	1194	WP_032434208.1	type II secretion system protein GspL	-
FLM33_RS09765	gspK	2006776..2007762 (-)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
FLM33_RS09770	gspJ	2007759..2008355 (-)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
FLM33_RS09775	gspI	2008352..2008717 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FLM33_RS09780	gspH	2008714..2009229 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=376924 FLM33_RS09745 WP_004213441.1 2003585..2004385(-) (pilD) [Klebsiella pneumoniae strain 89-17]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=376924 FLM33_RS09745 WP_004213441.1 2003585..2004385(-) (pilD) [Klebsiella pneumoniae strain 89-17]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425