Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   FQZ25_RS12095 Genome accession   NZ_CP042270
Coordinates   2309246..2312734 (+) Length   1162 a.a.
NCBI ID   WP_152305257.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain GA-A07     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2304246..2317734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQZ25_RS12080 (FQZ25_12090) lepB 2304815..2305378 (+) 564 WP_000751919.1 signal peptidase I -
  FQZ25_RS12085 (FQZ25_12095) addB 2305495..2309010 (+) 3516 WP_060852344.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FQZ25_RS27855 (FQZ25_12100) addA 2309092..2309277 (+) 186 WP_346726393.1 UvrD-helicase domain-containing protein Machinery gene
  FQZ25_RS12095 (FQZ25_12105) addA 2309246..2312734 (+) 3489 WP_152305257.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FQZ25_RS12100 (FQZ25_12110) - 2312747..2313034 (+) 288 WP_086692702.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  FQZ25_RS12105 (FQZ25_12115) gerPF 2313172..2313387 (-) 216 WP_001141566.1 spore germination protein GerPF -
  FQZ25_RS12110 (FQZ25_12120) - 2313430..2313816 (-) 387 WP_000902324.1 spore germination protein GerPE -
  FQZ25_RS12115 (FQZ25_12125) gerPD 2313832..2314026 (-) 195 WP_001052807.1 spore germination protein GerPD -
  FQZ25_RS12120 (FQZ25_12130) gerPC 2314033..2314647 (-) 615 WP_001070760.1 spore germination protein GerPC -
  FQZ25_RS12125 (FQZ25_12135) gerPB 2314715..2314921 (-) 207 WP_001012508.1 spore germination protein GerPB -
  FQZ25_RS12130 (FQZ25_12140) gerPA 2314936..2315157 (-) 222 WP_001111187.1 spore germination protein GerPA -
  FQZ25_RS12135 (FQZ25_12145) - 2315254..2315433 (-) 180 WP_000462841.1 aspartyl-phosphate phosphatase Spo0E family protein -
  FQZ25_RS12140 (FQZ25_12150) - 2315681..2316580 (+) 900 WP_001213071.1 fumarylacetoacetate hydrolase family protein -
  FQZ25_RS12145 (FQZ25_12155) - 2316617..2316898 (-) 282 WP_086692700.1 hypothetical protein -

Sequence

Protein


Download         Length: 1162 a.a.        Molecular weight: 134112.03 Da        Isoelectric Point: 4.7612

>NTDB_id=376482 FQZ25_RS12095 WP_152305257.1 2309246..2312734(+) (addA) [Bacillus thuringiensis strain GA-A07]
MGEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGI
EDNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQH
IRKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDE
VKKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVAL
QYRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGDGMKIDLA
KNFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARL
MAQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVID
NPMQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSD
LIWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMT
IHKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALT
RAKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSW
KVEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDN
AFIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFD
SDLGKRVLATKSVEREVPFTMMLAAEEAYQDWQGKSEEMILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPI
LEDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3489 bp        

>NTDB_id=376482 FQZ25_RS12095 WP_152305257.1 2309246..2312734(+) (addA) [Bacillus thuringiensis strain GA-A07]
ATCGGAGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAA
AGCTTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTT
TCCGTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATC
GAAGATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTT
AGCGCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAG
GAAAGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCAT
ATTCGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCATT
ACTTGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGTGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGT
TAAAGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAA
GTGAAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATT
AGAAAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCA
CAGATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTC
CAATATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGACACGAACTTTGTACAGGAATCCATTATTAAATT
CGTAACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAG
CCGAGCCAGGCTTATTCCTAGGAAAGTATAAACGTTTCACACAAGAAGGATTGGGCGACGGAATGAAGATTGACTTAGCG
AAAAACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGA
AATTGACTACGATGCTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTATTAT
GTATTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTT
ATGGCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGTCCGGTGCA
ATATCGCGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTC
CAGTATACGCTGACCTCGCGACTGGTTATTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGAT
AATCCGATGCAAGATATTCCGCTTGCCGCTGTACTTCGTTCCCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCT
TCGTGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAG
AACTTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGAT
TTAATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAAGCAAA
CTTACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGTTTTATTG
AACGTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTAGGTGAACAAGAAGACGTTGTTCGCATTATGACG
ATTCATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAAT
GAAGCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACAT
TATCACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTGACG
CGTGCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGA
ACATAGTGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATA
GACACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGATATGACACTAGCTGG
AAAGTAGAAGTTGTGGACGGTAACACACTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACT
TCGTGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATG
AGGATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAAC
GCCTTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCG
CGGAACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTG
CTGGAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGAC
AGTGACTTAGGTAAAAGGGTATTAGCGACGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGA
AGCATATCAAGATTGGCAAGGGAAGAGTGAAGAAATGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAG
ATGGCATTACGTTAATCGACTTTAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATT
TTAGAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAAAAAAGCTTACAACATCCTGTAAAAGAGAA
ATGTTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(4-386)

UvrD-like helicase C-terminal domain (UvrD_C)

(397-811)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(981-1152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

51.58

100

0.52

Multiple sequence alignment