Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HAP53_RS07990 Genome accession   NZ_CP049979
Coordinates   1694413..1694853 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain IBS28     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1689413..1699853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAP53_RS07970 (HAP53_07970) coaE 1689903..1690523 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HAP53_RS23610 - 1690548..1690592 (+) 45 WP_120795372.1 protein YacM -
  HAP53_RS07975 (HAP53_07975) guaC 1690748..1691791 (+) 1044 WP_001217338.1 GMP reductase -
  HAP53_RS07980 (HAP53_07980) hofC 1691826..1693028 (-) 1203 WP_000157234.1 protein transport protein HofC -
  HAP53_RS07985 (HAP53_07985) pilB 1693018..1694403 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  HAP53_RS07990 (HAP53_07990) pilA 1694413..1694853 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  HAP53_RS07995 (HAP53_07995) nadC 1695056..1695949 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HAP53_RS08000 (HAP53_08000) ampD 1696037..1696588 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HAP53_RS08005 (HAP53_08005) ampE 1696585..1697439 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HAP53_RS08010 (HAP53_08010) aroP 1697482..1698855 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=376016 HAP53_RS07990 WP_000360902.1 1694413..1694853(-) (pilA) [Escherichia coli strain IBS28]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=376016 HAP53_RS07990 WP_000360902.1 1694413..1694853(-) (pilA) [Escherichia coli strain IBS28]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377