Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   FP477_RS12620 Genome accession   NZ_CP042046
Coordinates   2493637..2494134 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain B2-7A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488637..2499134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP477_RS12595 (FP477_12595) gcvPB 2488799..2490271 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  FP477_RS12600 (FP477_12600) gcvPA 2490264..2491610 (-) 1347 WP_000019692.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  FP477_RS12605 (FP477_12605) gcvT 2491630..2492721 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  FP477_RS12610 (FP477_12610) - 2492880..2493404 (-) 525 WP_001015120.1 shikimate kinase -
  FP477_RS12615 (FP477_12615) - 2493394..2493540 (-) 147 WP_001792109.1 hypothetical protein -
  FP477_RS12620 (FP477_12620) comGF 2493637..2494134 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  FP477_RS12625 (FP477_12625) comGE 2494052..2494351 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  FP477_RS12630 (FP477_12630) comGD 2494338..2494784 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  FP477_RS12635 (FP477_12635) comGC 2494762..2495073 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  FP477_RS12640 (FP477_12640) comGB 2495087..2496157 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  FP477_RS12645 (FP477_12645) comGA 2496129..2497103 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  FP477_RS12650 (FP477_12650) - 2497155..2497778 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  FP477_RS12655 (FP477_12655) - 2497775..2498104 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  FP477_RS12660 (FP477_12660) - 2498104..2499090 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=375662 FP477_RS12620 WP_001788897.1 2493637..2494134(-) (comGF) [Staphylococcus aureus strain B2-7A]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=375662 FP477_RS12620 WP_001788897.1 2493637..2494134(-) (comGF) [Staphylococcus aureus strain B2-7A]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment