Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AAZO_RS09355 Genome accession   NC_014248
Coordinates   2051434..2053446 (-) Length   670 a.a.
NCBI ID   WP_013191066.1    Uniprot ID   D7DWD2
Organism   'Nostoc azollae' 0708     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2046434..2058446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAZO_RS09335 (Aazo_1966) bioU 2047123..2048118 (+) 996 WP_013191062.1 (S)-8-amino-7-oxononanoate synthase BioU -
  AAZO_RS09340 (Aazo_1967) - 2048163..2048724 (-) 562 Protein_2101 hypothetical protein -
  AAZO_RS09345 (Aazo_1968) - 2048836..2050059 (-) 1224 WP_013191063.1 type II secretion system F family protein -
  AAZO_RS09350 (Aazo_1969) pilT 2050197..2051321 (-) 1125 WP_013191064.1 type IV pilus twitching motility protein PilT Machinery gene
  AAZO_RS09355 (Aazo_1971) pilB/pilB1 2051434..2053446 (-) 2013 WP_013191066.1 GspE/PulE family protein Machinery gene
  AAZO_RS35185 (Aazo_1972) - 2053852..2054004 (-) 153 WP_013191067.1 hypothetical protein -
  AAZO_RS09360 (Aazo_1973) grpE 2054098..2054769 (+) 672 WP_013191068.1 nucleotide exchange factor GrpE -
  AAZO_RS09365 (Aazo_1974) - 2055190..2055513 (+) 324 WP_013191069.1 hypothetical protein -
  AAZO_RS09370 (Aazo_1975) dnaK 2055630..2057618 (+) 1989 WP_013191070.1 molecular chaperone DnaK -
  AAZO_RS09375 (Aazo_1977) - 2058049..2058267 (-) 219 WP_013191071.1 WD40 repeat domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75418.33 Da        Isoelectric Point: 5.5329

>NTDB_id=37561 AAZO_RS09355 WP_013191066.1 2051434..2053446(-) (pilB/pilB1) ['Nostoc azollae' 0708]
MTYSSPQRTSTALTTKTQFSPFGNHLVQSGYVNTEQMKQALIESRKSGKKLIDVLELITGQQLSAEFIREHKKQHLFELK
ILYGVESVDPEVNQIGNLMVGQLIDSLIPVDVCRRHRLVPLWKREDQIPPYVLVAMVEPDNLDASDDLNRILRNKNLSLQ
RMVITQEDYQHLINKYLDELAIREKEKEQKRDTDINQDIKYLEDQDIEEFGEDKDADINASIKDAEDAPIIKLVNRILFK
ALQEKVSDIHIEPQEENLRIRFRKDGVLHEAFDPVPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLHIVQDIVSKPFGLILVTGPTGSGKTTSLYSALAEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSQCRIPYTPTTEELARYGLSASKETSVTFYKANSLPPESIAEAKTKNEICGACNG
IGYKGRCGVYEVMRVTENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCKTCDATLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=37561 AAZO_RS09355 WP_013191066.1 2051434..2053446(-) (pilB/pilB1) ['Nostoc azollae' 0708]
ATGACTTACTCGTCACCACAACGGACTAGCACCGCGTTAACTACAAAAACACAGTTTTCGCCCTTTGGCAACCACCTAGT
GCAATCTGGCTATGTCAACACTGAACAGATGAAACAGGCACTAATAGAGAGCCGGAAATCTGGCAAGAAGCTGATAGATG
TCCTAGAGTTAATTACTGGGCAACAACTATCAGCAGAGTTTATTCGGGAACACAAGAAACAACATCTATTTGAACTAAAA
ATATTATATGGCGTTGAATCAGTAGATCCAGAAGTCAATCAAATTGGCAATCTGATGGTTGGACAATTGATTGATAGTCT
GATACCAGTCGATGTCTGTCGTCGTCATCGTTTGGTACCGCTATGGAAACGGGAAGACCAAATACCACCTTATGTTTTAG
TGGCAATGGTGGAACCAGATAATCTGGATGCTTCTGATGACCTGAACCGAATCTTGCGTAACAAAAACTTATCCTTACAG
CGGATGGTGATTACCCAAGAAGATTACCAGCATCTCATCAACAAATATTTAGATGAGTTGGCTATTCGGGAAAAGGAAAA
AGAACAAAAAAGAGATACTGATATTAATCAGGATATAAAATATCTGGAAGATCAGGATATAGAAGAATTTGGTGAGGACA
AGGATGCTGATATCAATGCATCAATTAAGGATGCTGAAGATGCACCCATTATCAAACTCGTCAACAGAATCCTGTTTAAA
GCTTTGCAAGAAAAGGTTTCGGATATTCATATCGAACCACAAGAGGAAAACTTACGCATTCGCTTCCGTAAGGATGGGGT
ACTGCATGAGGCTTTTGACCCTGTACCTAAAAAAATCATTCCGGCGGTGACAGCCCGATTTAAAATCATTTCCAATCTTG
ACATCGCGGAAAGGCGTTTACCTCAAGATGGACGCATCCGTCGTTTATTTGAAGGACGGAAAGTTGACTTCCGGGTGAAT
ACCTTACCCAGTCGCTATGGGGAAAAGGTGGTGTTACGGATTCTGGACAACTCTTCTACCCAACTAGGGTTGAATAAGTT
AATTACTGATCCAGAGACTTTGCATATTGTCCAGGATATCGTCAGCAAGCCCTTTGGCTTGATTTTGGTAACTGGTCCGA
CTGGTTCTGGTAAAACGACTTCGTTGTATTCGGCATTGGCAGAAAAGAACGATCCAGGAATTAACATTAGTACGGTAGAA
GACCCGATTGAATATAGTTTGCCAGGGATTACCCAAGTACAGGTAATTCGGGAAAAAGGGTTGGATTTTTCCACAGCATT
GCGGGCGTTCTTGCGTCAAGATCCCGATGTGTTGCTTGTGGGTGAAACACGGGATAAAGAAACGGCAAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTAGTATTAACTACCTTACACACTAATGATGCGCCTGGTGCGATCGCACGTCTAGGAGAA
ATGGGAATTGAACCCTTCATGGTTTCCAGTTCCCTAATTGGTGTTTTGGCACAACGTTTAGTGCGTCGAGTTTGTTCACA
ATGTCGAATTCCCTACACTCCCACTACTGAAGAACTGGCTCGTTATGGTCTGTCAGCATCCAAAGAAACATCAGTAACTT
TTTACAAGGCTAATTCCTTACCTCCAGAATCTATAGCAGAAGCTAAAACCAAAAATGAAATCTGTGGGGCTTGTAATGGC
ATTGGGTATAAAGGACGTTGTGGTGTTTATGAAGTTATGCGAGTCACAGAAAACCTCCAAACTCTCATCAACCAAGAAGC
ACCCACAGAACGCATTAAAGAAGTAGCTGTAGAAGAAGGTATGAAAACCTTGCTTGCTTACAGTCTAGATTTAGTGCGTC
AAGGTTCTACAACTCTGGAGGAAGTAGAGCGCGTAACCTTTACTGATACAGGTTTGGAAGCTGAATTAAAAGCCAAACGT
AAGAGTGGTCTAACTTGTAAAACTTGTGATGCCACATTACAACCAGAATGGCTCGATTGTCCCTACTGTATGACACCTCG
TTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DWD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.988

100

0.642

Multiple sequence alignment