Detailed information NTG0037528

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: TRAD_RS11635
• Genome accession: NC_014221
• Coordinates: 2543354..2546005 (+)
• Length: 883 a.a.
• NCBI ID: WP_013178811.1
• Uniprot ID: D7CSM8
• Organism: Truepera radiovictrix DSM 17093
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2538354..2551005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TRAD_RS15770 (Trad_2331)	-	2538644..2539240 (-)	597	WP_013178804.1	septal ring lytic transglycosylase RlpA family protein	-
TRAD_RS11605 (Trad_2332)	-	2539277..2539597 (-)	321	WP_041947273.1	hypothetical protein	-
TRAD_RS11610 (Trad_2333)	-	2539830..2540606 (+)	777	WP_013178806.1	TatD family hydrolase	-
TRAD_RS11615 (Trad_2334)	-	2540593..2541015 (-)	423	WP_013178807.1	hypothetical protein	-
TRAD_RS16375 (Trad_2335)	-	2541548..2541790 (+)	243	WP_041947274.1	hypothetical protein	-
TRAD_RS11625 (Trad_2336)	-	2541787..2542728 (+)	942	WP_013178809.1	alpha/beta hydrolase	-
TRAD_RS11630 (Trad_2337)	-	2542782..2543372 (+)	591	WP_013178810.1	YqeG family HAD IIIA-type phosphatase	-
TRAD_RS11635 (Trad_2338)	pilF	2543354..2546005 (+)	2652	WP_013178811.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
TRAD_RS11640 (Trad_2339)	pilT	2546043..2547194 (+)	1152	WP_013178812.1	type IV pilus twitching motility protein PilT	Machinery gene
TRAD_RS16645	-	2547522..2549063 (+)	1542	WP_245523540.1	CHASE4 domain-containing protein	-
TRAD_RS16650	-	2548960..2549313 (+)	354	Protein_2336	GGDEF domain-containing protein	-
TRAD_RS16655	-	2549335..2550156 (+)	822	WP_245523541.1	EAL domain-containing protein	-
TRAD_RS11650 (Trad_2341)	-	2550153..2550929 (+)	777	WP_148221243.1	hypothetical protein	-

Sequence

Protein

• Length: 883 a.a.
• Molecular weight: 96468.67 Da
• Isoelectric Point: 5.1049

>NTDB_id=37528 TRAD_RS11635 WP_013178811.1 2543354..2546005(+) (pilF) [Truepera radiovictrix DSM 17093]
MAVLSIGDKRLGAMLLERGFISDEGLQRAIARQAETGGRLSEVLINLGLLSEQRIARAIEEAMGVPLVHLTRTPTDPQAL
AKVPAALALELRALPFALQGTRLRVAFEDPLDALAAEELEDVSGCIVEVFQALGSELTWALATHYPELGLTPPAELNPAL
GSRVGELALTLGLITREQLEAALAQQARTGGLLGSILQEQGALSDVQLAELLAQQADLPFTADLRGARISERLASHLLRV
DALQYRAVPYQEEADGLVVALADPRRADDLREIIRTPVRFVVAPESQVQALTDALYEHDKGRLGETLLQAGKLRREQLRE
ALYEQQKRGRVRPLGDILVDLGFVSREDVQWALAEQRTRGGRLEDTLVQAGRISPEMLSRSLALQLGYAFVEENVQVDAA
AVALVPEGAVRRYNVMPLRVEGNTLTLAMKDPRHVFAIDDIRLLTGKEIRPAVATEETLIKLINRHYKSNENMEELAKAV
LEEIGDQDEIEDDSHLDDTALVKVVNNIIREAVFNDISDIHIEPYPDKVLVRVRKDGALREYMSLPRGVAGAVAARIKIM
GRLNIAERRLPQDGRVRFKDKNLEVDLRLSTLPTVYGEKVVMRLLKRASAIPDIEQLGFADYNFERFTDTIQKPYGIFLI
TGPTGSGKSFTTFSILKRIATPDVNVTTVEDPVEYEIPGINQTQVNVKAGLTFASALRAFLRQDPDVIMIGEIRDTETAK
IAVEAALTGHLVIATLHTNDATGAVTRLAEMGVEPFNISASLLGVLAQRLVRKVCAACRVAYTPDVDTLRRLGLAPEALQ
GATLYRGVGCTQCGGSGYNGRHAIHELFTVDAAAQRAIVQGRSSSELKELAVAAGMKTLRDDGVFKALAGITTLEEVLAR
TTD

Nucleotide

• Length: 2652 bp

>NTDB_id=37528 TRAD_RS11635 WP_013178811.1 2543354..2546005(+) (pilF) [Truepera radiovictrix DSM 17093]
GTGGCCGTCCTGTCAATAGGCGACAAACGCCTCGGCGCCATGCTTTTAGAGCGCGGCTTTATCAGCGATGAGGGGTTGCA
ACGCGCCATCGCGCGCCAGGCCGAAACGGGGGGGCGCCTCTCGGAGGTGCTCATCAACCTGGGGCTCTTGTCCGAGCAGC
GCATCGCGCGCGCCATCGAGGAGGCGATGGGGGTGCCGCTGGTGCACCTGACCCGCACCCCCACCGACCCCCAAGCGCTC
GCCAAGGTCCCGGCGGCGCTCGCTCTTGAGCTGCGCGCGCTGCCCTTCGCCCTGCAGGGGACGCGCCTACGGGTCGCTTT
CGAGGACCCTTTAGACGCGCTCGCCGCCGAGGAGCTCGAGGACGTCAGCGGCTGCATCGTCGAGGTCTTTCAGGCGCTCG
GCAGCGAACTCACCTGGGCGCTCGCCACCCACTACCCCGAACTCGGCCTCACCCCCCCCGCCGAACTCAACCCCGCGCTG
GGGTCGCGCGTCGGCGAGCTCGCGCTCACCTTAGGGCTCATCACCCGCGAGCAGCTCGAGGCCGCGTTAGCGCAGCAGGC
GCGTACGGGGGGGCTTTTGGGCAGCATCTTGCAGGAGCAGGGGGCGCTAAGCGACGTGCAGCTCGCCGAACTGCTCGCGC
AGCAAGCCGACCTGCCCTTTACCGCCGACCTGCGCGGCGCGCGTATCAGCGAACGGCTCGCCTCGCACCTGCTGCGCGTC
GACGCGCTGCAGTACCGCGCGGTGCCCTACCAAGAGGAGGCGGACGGTCTTGTCGTGGCGCTCGCCGATCCGCGCCGCGC
GGACGACCTTCGGGAGATTATCCGCACCCCCGTGCGCTTCGTCGTCGCCCCGGAGAGCCAGGTGCAGGCGCTCACCGACG
CCCTTTACGAACACGACAAGGGGCGGCTCGGCGAGACGTTGCTGCAAGCGGGCAAGCTGCGGCGCGAACAGCTGCGCGAG
GCGCTCTACGAGCAGCAAAAGCGCGGGCGCGTAAGGCCGCTCGGCGACATCTTGGTCGACCTCGGCTTTGTCAGCCGCGA
GGACGTGCAGTGGGCGCTCGCCGAGCAGCGCACGCGCGGGGGGCGGCTCGAGGACACCCTCGTGCAAGCGGGGCGCATCA
GCCCCGAGATGCTCTCGCGCAGCCTCGCGCTGCAACTCGGCTACGCCTTCGTCGAGGAGAACGTGCAGGTCGACGCGGCG
GCGGTCGCGCTCGTCCCCGAGGGGGCGGTGCGCCGCTACAACGTGATGCCGCTGCGCGTCGAGGGCAACACCCTCACGCT
CGCCATGAAAGACCCGCGGCACGTCTTCGCCATCGACGACATCCGGCTCCTCACGGGTAAAGAGATCCGCCCCGCGGTCG
CTACCGAAGAGACCCTCATTAAGCTGATCAACCGCCACTACAAGAGCAACGAGAACATGGAGGAGCTCGCCAAGGCGGTT
TTAGAGGAGATCGGCGACCAAGACGAGATTGAAGACGACTCCCACTTAGACGACACCGCGCTCGTCAAGGTCGTCAACAA
CATCATCCGCGAGGCGGTCTTCAACGACATCTCGGACATCCACATCGAACCCTACCCCGACAAGGTGCTCGTGCGGGTCC
GCAAAGACGGCGCGCTCCGCGAGTACATGTCGTTGCCGCGCGGCGTCGCCGGCGCGGTCGCGGCGCGCATCAAGATCATG
GGGCGCCTTAATATCGCCGAGAGGCGGCTGCCGCAAGACGGGCGGGTGCGCTTTAAAGACAAGAACCTCGAGGTCGACCT
GCGCCTCTCGACGCTGCCCACGGTCTACGGCGAAAAGGTCGTGATGCGCCTTTTAAAGCGCGCCTCGGCAATTCCCGACA
TCGAGCAGCTCGGTTTCGCCGACTACAACTTCGAGCGCTTTACCGACACCATCCAAAAGCCCTACGGCATTTTTCTCATC
ACCGGGCCGACCGGCTCGGGCAAGTCGTTTACCACCTTCTCCATCTTAAAGCGCATCGCCACCCCGGACGTCAACGTCAC
GACGGTCGAGGACCCCGTCGAGTACGAGATCCCGGGGATCAACCAGACGCAGGTCAACGTCAAAGCGGGGCTCACCTTCG
CGAGCGCCCTGCGCGCGTTTTTACGCCAAGACCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGAAACCGCCAAG
ATCGCCGTCGAGGCGGCGCTGACCGGCCACCTGGTGATCGCCACGCTGCACACCAACGACGCCACGGGGGCGGTCACGCG
GCTCGCGGAGATGGGGGTCGAACCCTTTAACATCTCCGCGTCGCTCTTGGGGGTGCTCGCGCAGCGGCTCGTGCGCAAGG
TGTGCGCCGCTTGCCGCGTCGCCTACACCCCCGACGTGGACACCCTGCGGCGTTTGGGGCTCGCCCCCGAAGCGCTCCAG
GGCGCGACGCTCTACCGCGGCGTCGGCTGCACGCAGTGTGGCGGCAGCGGCTACAACGGCCGCCACGCGATCCACGAACT
GTTTACCGTCGACGCCGCGGCGCAGCGCGCCATCGTGCAGGGCCGCTCGTCGAGCGAACTCAAAGAGCTCGCGGTCGCTG
CGGGGATGAAGACGCTGCGCGACGACGGGGTGTTCAAGGCGCTCGCCGGCATCACCACGCTCGAAGAGGTGCTCGCGCGC
ACCACCGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (507-773)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-147)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (197-300)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (367-471)

3D structure

• AlphaFold DB: D7CSM8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	56.887	100	0.575
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	54.576	100	0.547