Detailed information NTG0374760

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FOM15_RS12375
• Genome accession: NZ_CP041927
• Coordinates: 2534914..2535714 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 18-2374
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2529914..2540714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOM15_RS12355 (FOM15_12350)	dksA	2530494..2530949 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
FOM15_RS12360 (FOM15_12355)	sfsA	2531112..2531828 (-)	717	WP_023342992.1	DNA/RNA nuclease SfsA	-
FOM15_RS12365 (FOM15_12360)	thpR	2531828..2532364 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
FOM15_RS12370 (FOM15_12365)	hrpB	2532437..2534866 (+)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
FOM15_RS12375 (FOM15_12370)	pilD	2534914..2535714 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
FOM15_RS12380 (FOM15_12375)	-	2535711..2536456 (-)	746	Protein_2390	type II secretion system protein N	-
FOM15_RS12385 (FOM15_12380)	-	2536446..2536931 (-)	486	WP_004151943.1	type II secretion system protein M	-
FOM15_RS12390 (FOM15_12385)	gspL	2536924..2538117 (-)	1194	WP_032428212.1	type II secretion system protein GspL	-
FOM15_RS12395 (FOM15_12390)	gspK	2538104..2539090 (-)	987	WP_077254844.1	type II secretion system minor pseudopilin GspK	-
FOM15_RS12400 (FOM15_12395)	gspJ	2539087..2539683 (-)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
FOM15_RS12405 (FOM15_12400)	gspI	2539680..2540045 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
FOM15_RS12410 (FOM15_12405)	gspH	2540042..2540557 (-)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=374760 FOM15_RS12375 WP_004145898.1 2534914..2535714(-) (pilD) [Klebsiella pneumoniae strain 18-2374]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=374760 FOM15_RS12375 WP_004145898.1 2534914..2535714(-) (pilD) [Klebsiella pneumoniae strain 18-2374]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425