Detailed information NTG0037380

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M301_RS02720
• Genome accession: NC_014207
• Coordinates: 580418..581290 (-)
• Length: 290 a.a.
• NCBI ID: WP_013147227.1
• Uniprot ID: D7DMX2
• Organism: Methylotenera versatilis 301
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 575418..586290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M301_RS02690 (M301_0521)	-	576222..577097 (+)	876	WP_013147221.1	ATP-binding protein	-
M301_RS02695 (M301_0522)	-	577110..578102 (+)	993	WP_013147222.1	Nudix family hydrolase	-
M301_RS02700 (M301_0523)	-	578092..578601 (-)	510	WP_013147223.1	copper chaperone PCu(A)C	-
M301_RS02705 (M301_0524)	-	578608..578820 (-)	213	WP_013147224.1	DNA gyrase inhibitor YacG	-
M301_RS02710 (M301_0525)	zapD	578942..579700 (-)	759	WP_013147225.1	cell division protein ZapD	-
M301_RS02715 (M301_0526)	coaE	579799..580413 (-)	615	WP_013147226.1	dephospho-CoA kinase	-
M301_RS02720 (M301_0527)	pilD	580418..581290 (-)	873	WP_013147227.1	prepilin peptidase	Machinery gene
M301_RS02725 (M301_0528)	-	581522..581902 (+)	381	WP_013147228.1	hypothetical protein	-
M301_RS02730 (M301_0529)	-	581945..582943 (-)	999	WP_013147229.1	polyprenyl synthetase family protein	-
M301_RS02735 (M301_0530)	rplU	583167..583532 (+)	366	WP_013147230.1	50S ribosomal protein L21	-
M301_RS02740 (M301_0531)	rpmA	583566..583826 (+)	261	WP_013147231.1	50S ribosomal protein L27	-
M301_RS02745 (M301_0532)	cgtA	584018..585106 (+)	1089	WP_013147232.1	Obg family GTPase CgtA	-
M301_RS02750 (M301_0533)	proB	585108..586271 (+)	1164	WP_013147233.1	glutamate 5-kinase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31796.91 Da
• Isoelectric Point: 8.5882

>NTDB_id=37380 M301_RS02720 WP_013147227.1 580418..581290(-) (pilD) [Methylotenera versatilis 301]
MFSGFSDISSLLQGNTTLFISISVIFGLMVGSFLNVVIHRLPKMMEREWHNNCLELQGKEITEATKYTLAHPRSACPKCG
HKITALENIPVISYLLLLKGKCSGCKTAISVRYPLIEALTGLLIGLVSWKFGYTSLTLFAWIFTFALIALTFIDFDTQLL
PDDITLPLLWLGLLLNLNNGFTDLKSAVIGAMAGYLILWSVYWAFKLVTGKEGMGYGDFKLLAALGAWFGWQLLPAVILL
SSVLGAVIGISLIVFTKRGREIPMPFGPFLAIGGIAALFLGPQLASYYLT

Nucleotide

• Length: 873 bp

>NTDB_id=37380 M301_RS02720 WP_013147227.1 580418..581290(-) (pilD) [Methylotenera versatilis 301]
ATGTTCAGCGGATTTTCTGATATTTCTAGTTTGCTACAAGGGAACACCACACTTTTTATTTCCATATCCGTTATTTTTGG
CTTAATGGTCGGCAGTTTTTTGAATGTGGTGATTCATAGATTACCCAAGATGATGGAGCGCGAATGGCATAACAACTGCC
TTGAACTGCAAGGCAAAGAGATTACAGAGGCGACCAAATACACCCTCGCCCACCCTCGTTCAGCTTGCCCAAAATGCGGG
CATAAAATTACCGCACTAGAAAATATTCCAGTCATTAGCTACCTACTCTTACTGAAAGGTAAATGTAGCGGCTGCAAAAC
CGCTATCAGCGTGCGCTACCCTCTAATTGAGGCGCTGACAGGCTTATTGATTGGCCTAGTCAGCTGGAAATTCGGCTACA
CCAGCCTGACTTTATTTGCATGGATATTCACTTTCGCACTGATAGCACTGACCTTTATTGACTTCGACACTCAACTATTA
CCTGATGACATCACACTGCCACTATTATGGCTGGGCCTGCTACTCAACCTTAACAATGGCTTTACTGACTTAAAATCTGC
GGTGATTGGCGCGATGGCTGGCTATCTTATTTTATGGTCAGTTTATTGGGCATTTAAATTAGTGACTGGTAAAGAAGGCA
TGGGTTATGGCGACTTTAAATTACTCGCGGCACTCGGCGCTTGGTTTGGCTGGCAATTACTACCTGCAGTGATTTTACTG
TCTTCAGTACTGGGCGCTGTCATCGGTATTAGTCTGATTGTGTTTACAAAACGTGGGCGTGAAATCCCCATGCCGTTTGG
TCCATTTTTAGCGATTGGCGGTATTGCAGCGCTGTTTTTAGGGCCGCAACTTGCCAGTTATTATTTAACTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (142-250)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-131)

3D structure

• AlphaFold DB: D7DMX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	54.225	97.931	0.531
pilD	Vibrio cholerae strain A1552	54.513	95.517	0.521
pilD	Acinetobacter baumannii D1279779	51.408	97.931	0.503
pilD	Acinetobacter nosocomialis M2	51.056	97.931	0.5
pilD	Neisseria gonorrhoeae MS11	51.661	93.448	0.483