Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G6Z99_RS21515 Genome accession   NZ_CP049201
Coordinates   4468747..4470132 (-) Length   461 a.a.
NCBI ID   WP_024224798.1    Uniprot ID   -
Organism   Escherichia coli strain PapRG-04-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4463747..4475132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6Z99_RS21485 (G6Z99_21475) mutT 4464194..4464592 (+) 399 WP_000736016.1 8-oxo-dGTP diphosphatase MutT -
  G6Z99_RS21490 (G6Z99_21480) yacG 4464682..4464879 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G6Z99_RS21495 (G6Z99_21485) zapD 4464889..4465632 (-) 744 WP_001194721.1 cell division protein ZapD -
  G6Z99_RS21500 (G6Z99_21490) coaE 4465632..4466252 (-) 621 WP_001269510.1 dephospho-CoA kinase -
  G6Z99_RS27105 - 4466277..4466321 (+) 45 WP_120795372.1 protein YacM -
  G6Z99_RS21505 (G6Z99_21495) guaC 4466477..4467520 (+) 1044 WP_032222088.1 GMP reductase -
  G6Z99_RS21510 (G6Z99_21500) hofC 4467555..4468757 (-) 1203 WP_088375710.1 protein transport protein HofC -
  G6Z99_RS21515 (G6Z99_21505) pilB 4468747..4470132 (-) 1386 WP_024224798.1 type II secretion system protein GspE Machinery gene
  G6Z99_RS21520 (G6Z99_21510) ppdD 4470142..4470582 (-) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  G6Z99_RS21525 (G6Z99_21515) nadC 4470784..4471677 (-) 894 WP_158121436.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6Z99_RS21530 (G6Z99_21520) ampD 4471765..4472316 (+) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6Z99_RS21535 (G6Z99_21525) ampE 4472313..4473167 (+) 855 WP_158121435.1 beta-lactamase regulator AmpE -
  G6Z99_RS21540 (G6Z99_21530) - 4473197..4474147 (-) 951 WP_001154423.1 family 43 glycosylhydrolase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50609.28 Da        Isoelectric Point: 6.5396

>NTDB_id=373218 G6Z99_RS21515 WP_024224798.1 4468747..4470132(-) (pilB) [Escherichia coli strain PapRG-04-4]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=373218 G6Z99_RS21515 WP_024224798.1 4468747..4470132(-) (pilB) [Escherichia coli strain PapRG-04-4]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAATTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGCCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGATTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACACTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCTATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36

Multiple sequence alignment