Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   G6G93_RS09390 Genome accession   NZ_CP049118
Coordinates   1914715..1915524 (-) Length   269 a.a.
NCBI ID   WP_187976014.1    Uniprot ID   -
Organism   Escherichia coli strain EC931     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1909715..1920524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6G93_RS09375 (G6G93_09430) gspD 1911172..1913232 (-) 2061 WP_000498848.1 type II secretion system secretin GspD -
  G6G93_RS09380 (G6G93_09435) gspC 1913262..1914221 (-) 960 WP_000135086.1 type II secretion system protein GspC -
  G6G93_RS09385 (G6G93_09440) gspS2 1914239..1914649 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  G6G93_RS09390 (G6G93_09445) pilD 1914715..1915524 (-) 810 WP_187976014.1 prepilin peptidase PppA Machinery gene
  G6G93_RS09395 (G6G93_09450) sslE 1915654..1920219 (-) 4566 WP_058905481.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29479.08 Da        Isoelectric Point: 8.3801

>NTDB_id=372643 G6G93_RS09390 WP_187976014.1 1914715..1915524(-) (pilD) [Escherichia coli strain EC931]
MFFDVFQQYPAAIPVLATIGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWSESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=372643 G6G93_RS09390 WP_187976014.1 1914715..1915524(-) (pilD) [Escherichia coli strain EC931]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATACCCGTCCTGGCTACCATCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGTCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCCG
CTTTAGGTAGTTGGGTGGGGCCGTTATCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

42.804

100

0.431
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372