Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   THERJR_RS01305 Genome accession   NC_014152
Coordinates   268656..270056 (+) Length   466 a.a.
NCBI ID   WP_198360500.1    Uniprot ID   -
Organism   Thermincola potens JR     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 263656..275056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THERJR_RS01295 (TherJR_0245) ltrA 265675..266943 (+) 1269 WP_041587637.1 group II intron reverse transcriptase/maturase -
  THERJR_RS16235 - 267085..267249 (-) 165 WP_157723893.1 hypothetical protein -
  THERJR_RS01300 (TherJR_0247) - 267604..268566 (+) 963 WP_013119161.1 PP2C family protein-serine/threonine phosphatase -
  THERJR_RS01305 (TherJR_0248) radA 268656..270056 (+) 1401 WP_198360500.1 DNA repair protein RadA Machinery gene
  THERJR_RS01310 (TherJR_0249) disA 270057..271136 (+) 1080 WP_013119163.1 DNA integrity scanning diadenylate cyclase DisA -
  THERJR_RS01315 (TherJR_0250) - 271229..271624 (-) 396 WP_013119164.1 hypothetical protein -
  THERJR_RS01320 (TherJR_0251) - 271833..272309 (+) 477 WP_013119165.1 CarD family transcriptional regulator -
  THERJR_RS01325 (TherJR_0252) - 272449..273591 (+) 1143 WP_013119166.1 PIN/TRAM domain-containing protein -
  THERJR_RS01330 (TherJR_0253) ispD 273635..274288 (+) 654 WP_049771673.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  THERJR_RS01335 (TherJR_0254) ispF 274279..274755 (+) 477 WP_013119168.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 49667.50 Da        Isoelectric Point: 7.2697

>NTDB_id=37224 THERJR_RS01305 WP_198360500.1 268656..270056(+) (radA) [Thermincola potens JR]
MLSAVGRIDRVSRVRVRFCCQECGFESLKWLGRCPSCESWNSFVEEPLPAKKGAAGSGNLQMSGQPVSISDVVTVEEIRY
CTGIGELDRVLGGGIVRGSLILIGGDPGIGKSTLGLQAASAISIKTGRVLYVSGEESIHQTKLRALRLGAVSPDLYIVSE
TSLEGILRHIEKLQPNLVVVDSIQTVFTEEVHAAPGGVSQVRECTAHLMRLAKSTGIPVFIVGHVTKEGYLAGPRVLEHM
VDTVLYFEGDRHHNFRVLRAVKNRFGSTNEIGVFEMGEHGLVPVANPSEIFLAERTPGATGSVVIPAVEGTRPVLVEVQA
LVSPSNFAAPRRMTKGIDHNRVSLLAAVLEKRVGLHLANQDIFVNVVGGVKLDEPAIDLGLVAAIASSLKEIPVNPQTLV
VGEVGLTGEVRAVGQLEKRVKEGAKLGFKHIIAPGGNKVQSLEVNGINIIPVKSVAEVMDIILGGD

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=37224 THERJR_RS01305 WP_198360500.1 268656..270056(+) (radA) [Thermincola potens JR]
ATGTTAAGTGCAGTTGGAAGGATTGATAGGGTGAGCCGGGTTAGAGTTCGATTCTGTTGTCAGGAATGCGGGTTTGAAAG
CCTGAAATGGCTTGGACGCTGTCCTTCTTGTGAATCTTGGAACAGCTTTGTTGAAGAACCGTTGCCTGCCAAGAAGGGTG
CTGCGGGAAGCGGGAATTTACAAATGTCCGGTCAGCCCGTTTCTATAAGCGATGTTGTAACGGTGGAAGAGATAAGATAC
TGTACCGGTATTGGGGAATTAGACCGGGTCCTCGGTGGTGGTATTGTTCGTGGTTCCCTCATATTGATTGGCGGCGACCC
GGGTATAGGCAAATCTACCCTGGGGCTGCAGGCGGCCAGCGCCATCAGTATAAAGACTGGCCGGGTTCTTTACGTTTCCG
GAGAAGAATCTATTCACCAGACTAAGCTGCGGGCGCTCCGGTTGGGGGCGGTTTCCCCGGACCTATACATAGTATCGGAA
ACGAGTTTAGAGGGAATCTTAAGACATATTGAAAAGTTACAGCCCAACCTGGTGGTGGTTGATTCAATACAAACAGTATT
TACGGAGGAAGTACATGCTGCTCCCGGCGGTGTCAGCCAGGTCAGAGAGTGCACGGCTCACCTGATGCGGTTGGCCAAAT
CAACAGGTATCCCCGTTTTTATCGTCGGGCATGTGACCAAAGAGGGGTACCTGGCGGGGCCACGGGTTCTGGAGCATATG
GTAGATACCGTCCTGTATTTTGAAGGTGACCGTCATCATAATTTCCGGGTATTAAGGGCCGTAAAAAACCGCTTTGGCTC
CACCAATGAAATTGGTGTTTTCGAAATGGGGGAACACGGTTTAGTTCCGGTAGCAAACCCGTCTGAAATTTTTTTGGCTG
AAAGAACTCCCGGTGCTACGGGATCTGTCGTGATTCCTGCCGTGGAAGGGACCAGACCTGTGTTGGTTGAGGTTCAGGCC
CTGGTTTCACCCAGCAATTTCGCTGCGCCCCGCCGGATGACCAAAGGAATAGACCACAATCGCGTTTCTTTATTGGCTGC
CGTATTGGAGAAACGCGTTGGCCTGCATTTGGCTAACCAGGACATATTTGTCAATGTTGTCGGCGGTGTTAAGCTGGATG
AACCGGCTATAGACCTGGGACTTGTGGCCGCTATAGCGTCCAGCCTGAAGGAGATCCCGGTCAATCCCCAGACGCTGGTA
GTGGGAGAAGTGGGGCTGACAGGTGAAGTAAGGGCTGTTGGGCAACTGGAAAAGCGGGTTAAAGAAGGGGCTAAACTTGG
CTTTAAGCATATAATTGCTCCGGGCGGGAATAAAGTCCAGAGCCTTGAGGTTAATGGGATTAATATAATTCCTGTCAAAT
CGGTGGCCGAAGTAATGGATATAATTTTGGGGGGTGATTAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-463)

AAA domain (AAA_25)

(86-229)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(19-45)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

60.044

98.283

0.59
  radA Streptococcus mitis NCTC 12261

54.185

97.425

0.528
  radA Streptococcus pneumoniae Rx1

54.185

97.425

0.528
  radA Streptococcus pneumoniae D39

54.185

97.425

0.528
  radA Streptococcus pneumoniae R6

54.185

97.425

0.528
  radA Streptococcus pneumoniae TIGR4

54.185

97.425

0.528
  radA Streptococcus mitis SK321

54.185

97.425

0.528

Multiple sequence alignment