Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FNA74_RS07685 Genome accession   NZ_CP041515
Coordinates   1337245..1338087 (+) Length   280 a.a.
NCBI ID   WP_064374606.1    Uniprot ID   A0A7H4N5B2
Organism   Klebsiella michiganensis strain KNU07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1321002..1347870 1337245..1338087 within 0


Gene organization within MGE regions


Location: 1321002..1347870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNA74_RS31465 - 1321002..1321478 (+) 477 WP_064374602.1 PTS system mannose/fructose/N-acetylgalactosamine-transporter subunit IIB -
  FNA74_RS07605 - 1321490..1322290 (+) 801 WP_014228290.1 PTS mannose/fructose/sorbose transporter subunit IIC -
  FNA74_RS07610 - 1322305..1323150 (+) 846 WP_048263552.1 mannose/fructose/sorbose PTS transporter subunit IID -
  FNA74_RS31300 - 1323282..1325969 (+) 2688 WP_064374603.1 glycoside hydrolase family 32 protein -
  FNA74_RS07620 gspC 1326252..1327109 (+) 858 WP_064374604.1 type II secretion system protein GspC -
  FNA74_RS07625 gspD 1327143..1329089 (+) 1947 WP_086073926.1 type II secretion system secretin GspD -
  FNA74_RS07630 gspE 1329095..1330594 (+) 1500 WP_048263549.1 type II secretion system ATPase GspE -
  FNA74_RS07635 gspF 1330599..1331819 (+) 1221 WP_048263548.1 type II secretion system inner membrane protein GspF -
  FNA74_RS07640 gspG 1331849..1332277 (+) 429 WP_042944533.1 type II secretion system major pseudopilin GspG -
  FNA74_RS07645 gspH 1332277..1332828 (+) 552 WP_064374605.1 type II secretion system minor pseudopilin GspH -
  FNA74_RS07650 gspI 1332825..1333190 (+) 366 WP_048263547.1 type II secretion system minor pseudopilin GspI -
  FNA74_RS07655 gspJ 1333187..1333786 (+) 600 WP_072065692.1 type II secretion system minor pseudopilin GspJ -
  FNA74_RS07660 gspK 1333783..1334769 (+) 987 WP_048263546.1 type II secretion system minor pseudopilin GspK -
  FNA74_RS07665 gspL 1334769..1335974 (+) 1206 WP_048263545.1 type II secretion system protein GspL -
  FNA74_RS07670 gspM 1336062..1336454 (+) 393 WP_267285777.1 type II secretion system protein GspM -
  FNA74_RS30945 - 1336537..1337183 (+) 647 Protein_1237 type II secretion system protein N -
  FNA74_RS07685 pilD 1337245..1338087 (+) 843 WP_064374606.1 prepilin peptidase Machinery gene
  FNA74_RS07690 nfsB 1338246..1338899 (-) 654 WP_014228307.1 oxygen-insensitive NAD(P)H nitroreductase -
  FNA74_RS07695 - 1339018..1339386 (-) 369 WP_014228308.1 MmcQ/YjbR family DNA-binding protein -
  FNA74_RS07700 - 1339386..1339967 (-) 582 WP_004848568.1 TetR/AcrR family transcriptional regulator -
  FNA74_RS07705 - 1340158..1341258 (+) 1101 WP_064374607.1 MBL fold metallo-hydrolase -
  FNA74_RS07710 - 1341291..1341632 (+) 342 WP_004848570.1 RamA family antibiotic efflux transcriptional regulator -
  FNA74_RS07715 - 1342058..1343407 (+) 1350 WP_143444314.1 glycoside hydrolase family 172 protein -
  FNA74_RS07720 - 1343467..1345167 (+) 1701 WP_049079263.1 glycoside hydrolase family 32 protein -
  FNA74_RS07725 - 1345186..1346580 (+) 1395 WP_064374608.1 MFS transporter -
  FNA74_RS07730 - 1346872..1347870 (+) 999 WP_064374609.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 30557.66 Da        Isoelectric Point: 8.8641

>NTDB_id=372216 FNA74_RS07685 WP_064374606.1 1337245..1338087(+) (pilD) [Klebsiella michiganensis strain KNU07]
MTLLVAFSSTYPIIWWFGLGATGAIVGSFLNVVICRLPVMLEKHWQREALLQLSLPSPEPTARFNLVLPHSRCPYCHTPV
AARDNIPLLSFLLLKGKARCCGAPISAQYPLVEAATAALFVLTARVFPPGLALCGAWVFVSFLLILAVIDYHRQLLPDLL
TLPLLWIGLLFNLQSYFVSLPQATIGAVAGYLCLWCLFWLFKLLTGKDALGYGDFKLLAALGAWLGWQALPHVVLLAAIS
GLLFAFVQRGGAKKDLNQPLAFGPWLALAGVANMLLFQGI

Nucleotide


Download         Length: 843 bp        

>NTDB_id=372216 FNA74_RS07685 WP_064374606.1 1337245..1338087(+) (pilD) [Klebsiella michiganensis strain KNU07]
ATGACATTATTAGTGGCTTTTTCATCCACGTATCCCATCATTTGGTGGTTTGGTCTTGGGGCGACAGGCGCTATCGTCGG
GAGTTTTCTGAATGTGGTGATCTGCCGCTTACCCGTAATGCTCGAGAAACACTGGCAGCGTGAGGCGTTGCTGCAGCTGT
CACTGCCTTCACCTGAACCCACGGCGCGCTTTAATCTGGTATTGCCGCACTCTCGTTGCCCATATTGTCATACTCCTGTT
GCCGCAAGGGATAATATACCTCTGCTGAGTTTCCTGTTGTTAAAGGGCAAGGCTCGCTGTTGCGGCGCGCCTATTTCCGC
GCAGTATCCGCTGGTTGAGGCGGCCACCGCTGCGCTGTTTGTCTTAACCGCACGTGTATTTCCGCCGGGGCTGGCGCTGT
GTGGCGCCTGGGTATTCGTCTCTTTTTTATTGATCCTGGCCGTTATTGATTATCACCGACAGCTGCTACCCGATCTCCTG
ACGCTGCCCTTATTGTGGATCGGCTTACTGTTTAATCTTCAGTCGTATTTTGTTTCGTTGCCGCAGGCGACGATTGGCGC
AGTGGCAGGATATCTCTGCTTGTGGTGCCTGTTCTGGCTGTTCAAGCTACTGACAGGCAAGGATGCGCTGGGCTATGGCG
ATTTTAAGCTACTGGCCGCGCTGGGGGCCTGGCTAGGCTGGCAAGCATTACCCCATGTGGTTTTACTGGCGGCGATAAGC
GGATTGCTGTTTGCGTTCGTACAGCGAGGAGGGGCAAAAAAAGATCTCAATCAGCCGCTGGCATTCGGCCCGTGGCTGGC
GTTAGCCGGTGTGGCAAATATGCTGCTATTTCAGGGTATTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H4N5B2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.06

95.357

0.496
  pilD Vibrio campbellii strain DS40M4

49.077

96.786

0.475
  pilD Neisseria gonorrhoeae MS11

47.791

88.929

0.425
  pilD Acinetobacter nosocomialis M2

43.496

87.857

0.382
  pilD Acinetobacter baumannii D1279779

43.089

87.857

0.379

Multiple sequence alignment